A research group discovers that a reagent can be used to form covalent cross-links between various types of atoms in both small molecules and proteins. They add the reagent to a mixture of 14C-lactose and a protein that is thought to have a specific binding site for lactose. What should they do next to draw a conclusion about whether the protein specifically binds lactose

Answers

Answer 1

Answer and Explanation:

Ideally, researchers would measure the stoichiometry of the mixture labeled with 14C-lactose and a protein. By measuring this stoichiometry, researchers should be able to find a concentration between lactose and protein of 1:1. This concentration is able to determine that the protein has specifically bound to lactose.

In summary, to determine whether the protein binds lactose, researchers need to calculate whether the concentration of these two elements within the mixture is 1:1.


Related Questions

A scientist places 25 mg of bacteria in a culture for an experiment and he finds that the mass of the bacteria triples every day. The mass of the bacteria in the culture on any given day is what percent of the mass of bacteria in the culture exactly one day prior

Answers

Answer:

300%

Explanation:

The mass of the bacterial culture is triples everyday, meaning that after one day the culture will have 300% of the mass as the culture one day prior.

1. Describe the purpose of mitosis.
2. Describe what is happening in each stage and what each stage looks like:
Prophase
Prometaphase
Metaphase
Anaphase
Telophase
Cytokinesis

Answers

Answer:

1) The purpose of mitosis is growth and repair of cells

Explanation:

Prophase:

-DNA condenses (supercoiled, chromatids are visible under light microscope);

-Nuclear membrane begins to disintegrate (by the end of prophase  it is completely gone)

-Centrioles move to opposite ends of the cell

-Splinde fibers start to form from the centrioles and grow

Metaphase:

-Spindle fibers, departing from both poles, are attached to each centromere, on opposite sides

-The spindle fibers pull the chromosomes back and forth until they align in a plane along the equator of the cell, which is called the equatorial plane

Anaphase:

-The spindle fibers shorten

-The centromeres of sister chromatids split

-Chromatids move apart, pulled by the spindle fibers

-Chromatids become chromosomes

-Half of the chromosomes move to one cell pole, half to the other

Telophase:

-The chromosomes reach the cell pole

-Nuclear membrane reforms

-Spindle fibers break down and disappear

-The chromosomes begin to uncoil, which makes them diffuse and less compact

Cytokinesis:

-The cell membrane pinches inward until the cell divides in two;

-There are two daughter cells with the same structures and number of chromosomes as the parent cell

-Chromosomes decondense

The nitrogen cycle is the using and reusing of nitrogen in an ecosystem. True or false?

Answers

Answer:

True

Explanation:

Nitrogen is a fundamental component of both inorganic and organic compounds, where it is the main constituent of biomolecules such as nucleic acids (DNA, RNA) and proteins. The nitrogen cycle refers to the biogeochemical processes by which nitrogen circulates between the components of an ecosystem, i.e., between organisms (like plants and decomposers), and non-living things (i.e., soil, water, air). This cycle consists of several processes which include, among others, nitrogen fixation (i.e., the process by which nitrogen in the atmosphere is converted into ammonia), nitrification (i.e., the oxidation of ammonia is oxidized into nitrite and subsequent transformation of nitrites into nitrates), denitrification (where nitrate is reduced), anaerobic ammonia oxidation and putrefaction.

The sun is located .................. of the solar system​

Answers

In the Milky way galaxy

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Based on the body structures of these animals, which two conclusions are scientifically sound?
Bassa
dog
gorilla
chimpanzee
frog
0 The skeletal structure of the gorilla and chimpanzee suggest a common evolutionary ancestry.
The dog and frog share a closer evolutionary ancestry than the gorilla and chimpanzee.
The dog is more closely related to the frog than it is to the chimpanzee.
All four organisms share a common vertebrate ancestor.

Answers

Answer:

(A) The skeletal structure of the gorilla and chimpanzee suggest a common evolutionary ancestry.

Explanation:

The vertebrate and many of the other parts of the skeletal structure of the gorilla and chimpanzee are alike. (and, by using a little background knowledge, you can figure this out by thinking; if humans share 98% of DNA with chimpanzees and about 96% of DNA with gorillas, then the two animals must share a lot of DNA as well.)

I hope this helps !!!

Answer:

1, 3, 4

Explanation:

The gorilla and the chimpanzee have very similar anatomy, so they are closely related.

There is a small resemblance between the dog and the frog, but nowhere close to the one shared with the gorilla and chimpanzee, so NO

the dog IS more closely related to the frog than the chimpanzee since it looks more similar

yes, all of them are vertebrates and therefore share a single vertebrate ancestor

Explain the rate of drainage of the soils.​

Answers

Answer:

water drain deep in the soil

Explanation:

when evaporation increase the temperature will also increase as well as the transportation will also increase

You will be evaluating the texture (size of soil particles) and the soil drainage rate (the amount of time it takes water to soak into the ground). understanding these characteristics will help you properly size your rain garden.

Ammonia produced in the intestines from the breakdown of proteins by bacterial enzymes is the primary source of plasma ammonia
Select one:
1.True

2.False

Answers

Answer:

True

Explanation

Glycogen is an important and quickly mobilized source of stored glucose. Glucose is mobilized for ATP generation in muscle in response to epinephrine, which activates Gs. How would inhibitors of cAMP phosphodiesterase affect glucose mobilization in muscle

Answers

Answer:

Glucose mobilization would continue

Explanation:

The GTP-bound alpha subunit (Gαs) is a subunit of the heterotrimeric G protein which is required for stimulating the cAMP-dependent pathway through the activation of the membrane-associated enzyme adenylyl cyclase, which in turn catalyzes the synthesis of the second messenger cyclic-3′,5′-adenosine monophosphate (cAMP) from ATP. Moreover, cAMP phosphodiesterase is an enzyme that hydrolyzes cAMP, thereby negatively regulating the levels of this second messenger, which is fundamental for the termination of the signal. In this case, the epinephrine signal initiates glycogen breakdown (glucose mobilization) in muscle cells. In consequence, if the cAMP phosphodiesterase enzyme is inhibited, the concentration of cAMP will persist high even beyond the termination of the signal, thereby glucose mobilization would continue.

Which element is necessary to make proteins for plant growth?
FAst

Answers

Answer:

Nitrogen (N), Phosphorus (P), and potassium (k)

why is the silkworm called a useful insect?

Answers

Silkworm is called a useful insect because it produces very expensive silk which is soft, elastic,shiny and rearing silkworm can impact greatly on county’s economy.
That’s why ,Silkworm are called as useful insect.

Amoeba sisters video recap Biomagnification

Answers

I suggest you watch the Amoeba Sisters video again, 1-2x and take notes.

Biomagnification is when toxins / heavy metal are present in small amounts in fish, but accumulate as you move into higher trophic levels. This is one reason why it can be toxic for humans to eat tertiary or quaternary consumers, such as swordfish, shark, etc.

Biomagnification refers to the presence of higher concentration of chemical toxins as a result of the accumulation of toxins in organisms.

What is biomagnigication?

Biomagnification is best explained as a condition in which the chemical concentration of a toxin is amplified in an organism compared to the environment in which the organism is found.

Biomagnification usually is observed as one goes higher in the trophic levels of organisms.

For example, chemical pollutant found in water may be present at tolerable levels. However, in organisms, living in the water, the concentration of the pollutant is higher as these organisms accumulate these toxins in their tissues.

Therefore, biomagnification refers to the accumulation of toxins in organisms higher than found in their environment.

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Rosita Sanchez is a 2 month pregnant and she and her doctor are worried because her rash was diagnosed as a—————-. They are concerned because this condition can produce defects in rosita’s
developing child

Answers

Answer:

Rubella

Explanation:

Rubella is an infectious disease caused by a virus referred to as rubivirus, which can be spread from pregnant women to their unborn children through the bloodstream. During the first trimester of pregnancy, rubella can cause the death of the developing fetus (i.e., miscarriage) or serious birth defects such as, for example, heart defects, cataracts, deafness, mental incapacities, etc. Rubella symptoms include, among others, rash, joint pain, swollen lymph glands (especially behind the ears), headache, and mild fever.

Name the two layers that make up the skin, and identify a function for each layer.

Answers

Dermis and epidermis
Epidermis help us to protect the inner organs and also the dermis
The epidermis, the outermost layer of skin, provides a waterproof barrier and creates our skin tone.

The dermis, beneath the epidermis, contains tough connective tissue, hair follicles, and sweat glands.

High blood pressure occurs when there is too much blood flowing through the blood vessels at one time. By changing the amount of water in the blood, blood pressure can change. The excretory system can help to lower blood pressure.

Which would most likely occur in the kidney?

More water would be transferred from the nephron.
Less water would be absorbed into the nephron.
The amount of water in the bladder would decrease.
The amount of water in the blood would increase.

Answers

Answer:

c

Explanation:

The kidney plays an essential role in regulating blood pressure by adjusting the volume and concentration of water and solutes in the blood. Therefore, to lower blood pressure, Less water would be absorbed into the nephron. Hence option B is correct.

What is a nephron?

The nephron is the functional unit of the kidney that is responsible for filtering waste and excess water from the blood. When there is an excess of water in the blood, the kidney can decrease the amount of water that is reabsorbed into the body.

This would result in more water being excreted from the body in the urine, reducing the volume of blood and lowering blood pressure.

To achieve this, the kidney reduces the secretion of antidiuretic hormone (ADH) or vasopressin, which controls water reabsorption in the kidneys.

This decrease in ADH secretion causes the nephrons to reduce the amount of water they reabsorb from the urine, leading to an increase in urine output and a decrease in blood volume.

Therefore option B Less water would be absorbed into the nephron is correct.

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which statement describes a process associated with meiosis

Answers

Answer:

homologous chromosomes pair up

Answer:

Haploid cells join to form an organism that has a complete set of chromosomes.

Explanation:

The organisms that harness non-biological energy and convert it to biologically relevant/useful energy are called __ 1 __ _. The organisms that consume these are called __ 2 __ _ (it should end in -ores) which occur at the ___ 3 ___ trophic level. The number of trophic levels that any ecological system will primarily dependent on the __4__ organisms.

Answers

Answer:

The organisms that harness non-biological energy and convert it to biologically relevant/useful energy are called __autotrophos or producers_. The organisms that consume these are called _herbivores_ (it should end in -ores) which occur at the __second_ trophic level. The number of trophic levels that any ecological system will primarily dependent on the _consumer_ organisms.

Explanation:

 In the trophic web occurs energy transference through organisms occupying different levels in the chain. Each level feeds on the preceding one and becomes food for the next one. The first link is occupied by autotroph organisms, which are the producer. The following links are the consumers: herbivores are primary consumers and feed on producers. Carnivores are secondary consumers and feed on herbivores, and so on. The last links are the decomposers, microorganisms that act on dead animals degrading organic matter.  

Every link has an effect on the superior links and the immediately anterior link, meaning that whenever one of the links changes, the other ones will be affected.  

Autotrophs or producers synthesize inorganic substances, such as light, and turn them into organic matter according to their own needs. These organisms are photoautotrophs, such as plants, or chemoautotrophs. They occur at the first trophic level.

Heterotrophs are those incapable of producing their own organic matter, so they feed on producers, depending on them to get proteins and energy. In the trophic chain, heterotrophic organisms occupy the first, second, or third consumer level, after producers. These animals can be herbivorous, carnivorous,  omnivorous, hematophagous, ichthyophagous, and etcetera. All of them depend on autotrophic organisms.

In the particular case of herbivores, they occur at the second throphic level feeding on producers and being eaten by carnivores.

In general, most trophic chains are composed of 4 or 5 levels, depending on the number of consumers present, and the energy transference between levels.

explain the charge distribution in polar molecule​

Answers

Answer:

polarity results from uneven partial charge distribution between atoms in a compound

Explanation:

electrons in a polar covalant bond are unequally shared between the two bonded atoms which results in partial positive and negative charges.the separation create a dipole.

What else is produced during the replacement reaction of silver nitrate and potassium sulfate?

2AgNO3 + K2SO4 Ag2SO4 + ________

KNO3
2KNO3
K2
2AgNO3

Answers

Answer:

2KNO3

Explanation:

Pls mark brainliest

hope it helps u

From the equation of reaction, the other product during the replacement reaction of silver nitrate and potassium sulfate is potassium nitrate, KNO₃.

What is produced in the replacement reaction silver nitrate and potassium sulfate?

In the replacement reaction of silver nitrate and potassium sulfate, the products are silver sulfate and potassium nitrate.

Silver sulfate is insoluble and comes out of solution as a precipitate.

The equation of the reaction is given below:

2 AgNO₃ + K₂SO₄  ----> Ag₂SO₄ + 2 KNO₃

Therefore, the other product during the replacement reaction of silver nitrate and potassium sulfate is potassium nitrate, KNO₃.

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Which one of the following physical quantities is formed after dividing mass of volume?
I} Weight
II} Velocity
III} Density
IV}Work​

Answers

Answer:

Density

Explanation:

Density is refer to how much space an object in Mass take in volume.

It is mass if an object divided by volume.

It usually in g/cm3


Question 19
How would the carbon cycle be influenced by an increase in aerobic respiration?

Answers

Answer:

Cellular respiration does not affect the carbon cycle but is part of the carbon cycle. The 2 carbon cycles. carbon ( C ) is taken up by the plants and trees in the form of carbon dioxide ( CO2 ). Plants converts this CO2 into organic compounds such as glucose ( C6H12O6 ).

Explanation:

Increase in aerobic respiration will lead to increase in the amount of CO2 released into to the atmosphere due to respiration.

Aerobic respiration occurs when oxygen is combined with glucose to yield carbon dioxide and water. This is one of the major ways through which carbon dioxide enters the carbon cycle.

Note that the carbon cycle shows the movement of carbon in nature. Increase in aerobic respiration means that more carbon will be introduced into the atmosphere as carbon dioxide.

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What is photosynthesis???? ​

Answers

Explanation:

The process of green plants make their own food by present sunlight is called photo synthesis

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Hey there!

Photosynthesis is the reaction that converts light energy to chemical energy in sugar and carbohydrates. Humans can't eat sunlight, so we eat plants. Plants convert the energy in the sunlight into chemical energy using chlorophyll. Chlorophyll is a molecule produced by plants, algae, and cyanobacteria, which aids in the conversion of light energy into chemical bonds. It is the green pigment found in the chloroplasts of higher plants. They are a part of our ecosystem, and they are lower on the food chain.

Hope this helps!

Have a great day! :)

What is the most abundant greenhouse gas?

Answers

Answer:

Water Vapor

Explanation:

The most abundant greenhouse gas overall, water vapor differs from other greenhouse gases in that changes in its atmospheric concentrations are linked not to human activities directly, but rather to the warming that results from the other greenhouse gases we emit. Warmer air holds more water.

Mention the four characteristics of warm blooded animals.​

Answers

Answer:

They maintain their body temperature higher than their environment by some metabolic processes like contraction of muscles, friction produced by flow of blood etc. They have a higher basal metabolic rate, and also a greater capacity to increase their metabolic rate.

How does energy acquisition in the deep sea differ from energy acquisition near the ocean’s surface?
a.
Organisms in the deep sea acquire energy directly from the sun.
b.
Organisms near the ocean’s surface rely on chemosynthesis rather than photosynthesis.
c.
Organisms in the deep sea do not have direct access to sunlight.
d.
Organisms in the deep sea have no means to obtain energy unless they travel to the ocean’s surface.

Answers

Answer:

Organisms in the deep sea acquire energy directly from the sun.

On the basis of their use and development, Natural resources can be classified as ________ and ________​

Answers

Answer:

renewable , non-renewable

Explanation: natural resources can be classified as either renewable or non-renewable depending on whether or not they replenish naturally.

A suspension of yeast cells is being grown under anaerobic conditions such that glucose is metabolized to ethanol and carbon dioxide. An inhibitor of alcohol dehydrogenase is added to the suspension, the cells rapidly die. Explain.

Answers

Answer:

The correct answer is - NAD needed to keep glycolysis going is not being recycled so the entire process is shut down and no ATP is produced

Explanation:

Alcohol dehydrogenase plays role in metabolizing the bulk of ethanol consumed as part of the diet and their activities contribute to the rate of ethanol elimination from the body.

Inhibitor of this zinc enzyme prevents it to perform its function which results in NADH generated during anaerobic conditions glycolysis cannot be recycled back to NAD+, So the ATP production pf anaerobic condition through glycolysis cannot be maintained.

scientists believe the earth likely warmed between 0.65 celsius and 1.05 celsius with an average value of 0.85 celsius between years 1880 and 2012. what was error bar for the increase in temperature over that period?

Answers

Answer:

The lower limit is 0.65°C

The upper limit is 1.05°C

the average is 0.85°C

The difference between the average and the lower limit is:

0.85°C - 0.65°C = 0.20°C

the difference between th average and the upper limit is:

0.85°C - 1.05°C = -0.20°C

Then we can write the change in temperature as:

T = 0.85°C ± 0.20°C

Now we want to find the error bar.

The error bar would be the total distance between the lowest value and the largest value.

Then the error bar is:

1.05°C - 0.65°C = 0.40°C

notice that the error bar is twice the error, which makes a lot of sense because the error (± 0.20°C) goes up and down.

Then the error bar in this measure has a magnitude of 0.40°C.

#Impossible......!!!!!!!!!!
.
.
What is ecosystem???? explain!!!!! ​

Answers

it is where plants, animals and other organisms work together to create life as it is

Using the Venn diagram (above) can you come up with some ways that the different perspectives
overlap? What do they all have in common? Write a few words of commonalities for each overlap in
the Venn diagram. Can you find one in common for all three?

Answers

Answer:

you have not posted any Venn diagrams, so it is of an impossible feat for anyone to assist in your problem

hi everyone,what's is climate change.hope its easy for u all think u​

Answers

Climate change includes both global warming driven by human-induced emissions of greenhouse gases and the resulting large-scale shifts in weather patterns.
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Could someone please answer this question for me meaning of 2nd stanza of the poem vocation A particle moves along line segments from the origin to the points (3, 0, 0), (3, 4, 1), (0, 4, 1), and back to the origin under the influence of the force field F(x, y, z) = z^2i + 4xyj + 5y^2k. Use Stokes' Theorem to find the work done. Which element and animal best match my personality? 13. When Sheila entered the room, Barbara in pain A) was groaning B) will groan C) were groaning D) cannot groan The Campbell Company is considering adding a robotic paint sprayer to its production line. The sprayer's base price is $1,080,000, and it would cost another $19,000 to install it. The machine falls into the MACRS 3-year class (the applicable MACRS depreciation rates are 33.33%, 44.45%, 14.81%, and 7.41%), and it would be sold after 3 years for $626,000. The machine would require an increase in net working capital (inventory) of $18,500. The sprayer would not change revenues, but it is expected to save the firm $436,000 per year in before-tax operating costs, mainly labor. Campbell's marginal tax rate is 30%. Cash outflows, if any, should be indicated by a minus sign. Do not round intermediate calculations. Round your answers to the nearest dollar.What is the Year-0 net cash flow? Susan knows that there are laws that govern the refilling of diazepam, which is a controlled substance. How do these laws affect Mr. Lehmke? What should Susan do to respond to this medication refill request? After talking with the patient, Dr. Penningworth has approved the request for one refill. What is Susan's next step? How did England benefit from the industrial revolution ? 2 cm = 7.5 m in the drawing the mall is 8.6 cm tall how tall is the actual mall Find the missing side round your answer to the nearest tenth amount of tax, TTT, in dollars to be paid by an individual with an income of III between 8{,}2258,2258, comma, 225 and 34{,}50034,50034, comma, 500 dollars is given by the equation above. Tax is paid from the portion of income above 8{,}2258,2258, comma, 225 dollars and the remaining amount becomes a part of disposable income. What percentage of income above 8{,}2258,2258, comma, 225 dollars remains after paying tax for an individual whose income falls within the specified range Drag each tile to the correct location.Match each punctuation mark to what it indicates.:( )PurposePunctuation Marksets off information that is helpful, but not always essentialarrowRightsets apart information that is essential to the meaning of a sentencearrowRightintroduces a list, a definition, an explanation, or a quote that follows an independent clausearrowRight We use __force to do different force. 1) pulling 2)pushing 3)gravity 4)muscular the air pressure at the base of the mountain is 75.0cm of mercury while at the top is 60cm of mercury. Given that theaverage density of air is 1.25kg/m and the density of mercury is 13 600 kg/mand gravity-10N/kg, calculate the height height of the mountain 3 is a polynomial of degree ________. 10 Explique ampliamentelas caractersticasprincipales delCristianismo durante laEdad Media Uma maneira de realizar a operao 9972 - 9 de forma rpida e correta calculando 1 000 * 994, que igual a 994 000. A igualdade que justifica esse fato est expressa em:a) (a + b) = a + b + 2ab//b) a - b = (a + b)(a - b)//c) a - b = (a - b)//d) (a - b) = a - 2ab + b//e) a - b = 1 000(a + b) The Laplace Transform of a function f(t), which is defined for all t > 0, is denoted by L{f(t)} and is defined by the improper integral L{f(t)}(s) = infinity 0 e-st.f(t)dt, as long as it converges. Laplace Transform is very useful in physics and engineering for solving certain linear ordinary differential equations. (Hint: think of as a fixed constant) 1. Find L{t}. (hint: remember integration by parts) A. 1 B. -1/s2 C. 0 D. 1/s2 E. -s2F. None of these2. Find L{1}.a.1/sb. 1c. 0d. -se. -1/sf. none of these Relative to a stationary observer, a moving clock Group of answer choices can do any of the above. It depends on the relative energy between the observer and the clock. always runs faster than normal. can do any of the above. It depends on the relative velocity between the observer and the clock. always runs slower than normal. keeps its normal time. 10) It takes 1 'A cups of sugar and 3 cups of flour to make Sarah's cookies. What is the ratio of sugar to cookies?