Step 1: Lysis
In this step, the cell and the nucleus are broken open to release the DNA inside and there are two ways to do this. First, mechanical disruption breaks open the cells. This can be done with a tissue homogenizer (like a small blender), with a mortar and pestle, or by cutting the tissue into small pieces. Mechanical disruption is particularly important when using plant cells because they have a tough cell wall. Second, lysis uses detergents and enzymes such as Proteinase K to free the DNA and dissolve cellular proteins.
Step 2: Precipitation
When you complete the lysis step, the DNA has been freed from the nucleus, but it is now mixed with mashed up cell parts. Precipitation separates DNA from this cellular debris. First, Na + ions (sodium) neutralize the negative charges on the DNA molecules, which makes them more stable and less water-soluble. Next, alcohol (such as ethanol or isopropanol) is added and causes the DNA to precipitate out of the aqueous solution because it is not soluble in alcohol.
Step 3: Purification
Now that DNA has been separated from the aqueous phase, it can be rinsed with alcohol to remove any remaining unwanted material and cellular debris. At this point, the purified DNA is usually re-dissolved in water for easy handling and storage.
The DNA should be perform in three steps that is Lysis Precipitation and purification .
What is the process of DNA ?First: Lysis
There are two ways to release the DNA from the interior of the cell and nucleus at this process. The cells are initially ruptured mechanically. A tissue homogenizer (which resembles a tiny blender), a mortar and pestle, or cutting the tissue into little bits can all be used for this. Plant cells have a strong cell wall, thus mechanical disruption is crucial when employing them.
Stage 2: Precipitation
The DNA has been released from the nucleus once the lysis process is complete, but it is now intermingled with broken-down pieces of cell. DNA is separated from this cellular waste by precipitation. The negative charges on the DNA molecules are first neutralized by Na + ions (sodium), making them moreless water-soluble and stable. The DNA precipitates out of the aqueous solution when alcohol (such as ethanol or isopropanol) is then added since it is not soluble in alcohol.
Third step: purification
DNA can now be washed with alcohol to eliminate any remaining undesirable material and cellular debris after being removed from the aqueous phase. To make handling and storage easier, the pure DNA is now typically redissolved in water.
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True/False - A person under 18 years of age may not operate a motor vehicle while using a wireless communications device, except in case of emergency.
Can a Sheriff arrest or detain? Give a full expalanation on what you choose and also explain what the sheriff must do of the answer you decide to choose.
Answer:
A sheriff can do both.
Explanation:
The sheriff is the chief he can Arrest and Detain.
Communication Styles
Which of the following communication styles can be referred to as a “broken record”?
A. Passive assertive
B. Passive aggressive
o
C. Passive
u
D. Assertive
QUESTION 4
Police officers have incredible decision-making authority (descretion) when dealing with citizens and suspects.
True
False
Answer:
false
Explanation
it depends are you saying all police officers have incredible decision making or some but if you're saying all, some police officers aren't really good at making incredible decisions
SOMEONE PLEASE HELP!
Wind has little effect over the control of you’re vehicle
True or false
sory no sabo la respuesta
The first coin I pull from my bag is a penny, the second coin I pull from my bag is a penny therefore I reason that all the coins in my bag are pennies. This is an example of
a Deductive reasoning
b Inductive reasoning
The first coin I pull from my bag is a penny, the second coin I pull from my bag is a penny therefore I reason that all the coins in my bag are pennies. This is an example of Inductive reasoning.
What is Inductive reasoning?The process of inductive reasoning involves moving from the particular to the general in order to arrive at conclusions. Deductive reasoning, where you proceed from broad information to specific conclusions, is typically contrasted with it. Bottom-up reasoning and inductive logic are other names for inductive reasoning.
A logical thought process called inductive reasoning draws conclusions from observations and experiential data. Inductive reasoning is the process of making decisions based on a particular collection of data or previously acquired information from experiences.
The process of inductive reasoning involves moving from the particular to the general in order to arrive at conclusions.
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Which object will require the greatest amount of force to slow it to a stop, assuming all objects are initially moving at the same speed?
a bicycle
a bowling ball
skateboard
Stephen has been sentenced to five years in prison after being found guilty of burglary. However, he has demonstrated extremely good behavior in the first two years of his sentence. Further, he has made great progress in his rehabilitation program. Considering the situation, the court has decided to give Stephen an early release from his prison sentence. Which alternative to imprisonment is seen in this example? A. probation B. diversion C. parole D. community service E. economic sanction
Answer:
The answer according to the severity of crime would most likely be probation.
Explanation:
The judge will grant probation and the inmate has to comply with the terms.
Answer:
probation
Explanation:
:)
Which type of government gets its power from the will of the people?
Answer:
popular sovereignty
Explanation:
Answer:
Democracy
Explanation:
Democracy is when people chose their own government. People chose the government at their own will.
When the Chicago Police Department raised the minimum age for an applicant from 21 to 25, they did so because
Answer:
they wanted a more mature police force
How long are police chiefs appointed to their jobs
Answer:
Among large police organizations, 21/2 to three years is now the average
How are Congress’s implied powers different from its expressed powers? make it short
Answer:
Enumerated powers are mentioned in the Constitution, implied powers are not but necessary for the government to do its job.
Explanation:
what does it take you to be a police officer or state trooper
Why is voting a good way to make decisions? It is a fair way to choose leaders. It is the quickest way to choose leaders. Only smart people are allowed to vote. It is the least expensive way to choose leaders.
Answer:
Voting is a fair way to choose leaders. Voting usually takes a while, but it is a better way to know what the representative plans to do when he/she has a certain role in government.
Explanation:
Supreme Court. Jim, who is a bit eccentric, says that he is fed up with the way a certain employer in his town treats employees and that he is going to sue that employer in an effort to improve matters. Jim also says that he is going to start his case at the appellate court level, skipping over all those "lesser" judges. Jim says that those justices will surely hear him out and that he will also seek a jury. Although he is not a lawyer, Jim believes that the offenses of the employer are so severe that the justices will appreciate his attempt to make things better for the employees involved. Regardless of what court is involved, can Jim act as plaintiff for the employees? Multiple Choice Yes, so long as he gives any money he receives to them No, because venue is lacking Yes, so long as they file no objection No, because he lacks standing Yes, so long as he gets permission slips from them
Answer: No, because he lacks standing
Explanation:
As a private citizen who is not a lawyer, Jim cannot represent his fellow employees as that would require being a lawyer. Were he representing himself alone, he would have been allowed to represent himself.
As a non-lawyer however, he cannot represent multiple people as plaintiff. He therefore has no legal standing to do so.
Lab safety breakout red stick
1.Know emergency exit routes.
2.Avoid skin and eye contact with chemical
3.minimize all chemical exposure
4.no horseplay will tolerate
5.assume that all chemicals of unknown toxicity are highly toxin
If u could join one federal agency which would it be and why
SOMEONE PLEASE HELP!
Answer:
The answer you have is correct it's true
1. There are two categories (shapes) for the Virginia driver's license. The
license represents the driver who is under the age of 21, and the
license represents the driver who is over the age of 21.
shape
shaped
Answer:
The Virginia driver's license for drivers who are under 21 years of age is portrait shaped
The Virginia driver's license for drivers who are over 21 years of age is landscaped shaped
Explanation:
The shape of the Virginia driver's license for drivers who are under 21 years of age and drivers who are above 21 years of age depends on the orientation of the image with respect to the dimensions of the printed photograph.
When the image is oriented such that when it is in the upright position, the width of the printout is shorter than the height, the printout or image shape is said to be in portrait form
When the image is oriented such that when it is in the upright position, the width of the printout is longer than the height of the printout, the printout or image shape is said to be in landscape form.
why do people find it that they have to have viloence in their lives
Answer:
Obstacles people face and people they meet change who they are and winds up getting them to a point in life to where they have to make a decision.
Explanation:
Hopefully this helps you :)
pls mark brainlest ;)
Which best explains how the Supremacy Clause is connected to federalism?
The Supremacy Clause establishes that the federal government has more power than state governments.
The Supremacy Clause establishes that the state governments have more power than the federal government.
O The Supremacy Clause forces the federal government and state governments to share power equally.
O The Supremacy Clause forces the federal government and state governments to fight for power
Answer: The Supremacy Clause establishes that the federal government has more power than state governments.
Supremacy clause is found in the constitution of the USA and establishes federal laws. There are certain treaties under the authority. It represents the supreme law of the land.
It is essential for a conflict of laws and rules specific that a federal act takes into focus over the state which can conflict with the federal laws. Hence the clause states that the federal has more power than the state govt.Hence the option A is correct.
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House leaders may use any of these calendars to schedule debate on a bill EXCEPT
a. Union Calendar.
b. House Calendar.
c. Congressional Calendar
d. Private Calendar.
Decribe what happen in the unclear law
Answer:
It is then to the respective court to determine what exactly it means. When a law is unclear, a judge interprets it and determines what? When the legislature passes unclear law or ambiguous law the judiciary is forced to decide what the law really means. Once the citizens have accepted judges deciding what the law really is, instead of the Congress passing clear law. The judiciary is empowered to interpret law.
Hope this helped you!
Explanation:
“Police and crime commissioners have no real power, their presence is to just keep the public happy” arguments for and against
Which statement correctly defines the concept of atavism?
A.
a tendency to attack others violently
B.
a tendency to return to an old ancestral phase and animalistic characteristics
C.
a tendency to adapt a primitive lifestyle and habits
D.
a tendency to imitate animals and learn their behavior
Answer:
B
Explanation:
trust me it is B :))))))))
Atavism is a tendency to return to an old ancestral phase and animalistic characteristics. Thus, the correct answer is option B.
What is atavism?
Atavism is an evolutionary term that refers to the reappearance of traits that had vanished generations before. In criminology, this can refer to people who share characteristics with someone who lived a long time ago. It is a tendency to return to something ancestral or ancient.
An atavism is a biological structure modification in which an ancestral genetic trait reappears after being lost through evolutionary change in previous generations.
Atavisms can occur in a variety of ways, one of which is when genes for previously existing phenotypic features are preserved in DNA and become expressed via a mutation that either knocks out the dominant genes for the new traits or causes the old traits to dominate the new ones.
Therefore, a tendency to return to an old ancestral phase and animalistic characteristics is termed as atavism.
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what is a perfect work environment like for thinkers? A. Challenging, lonley and partially empty B. full of problems to solve and small See relax enough to be efficient and find shortcuts to systems
Did you anticipate the internal organizational consequences based on your choices?
I
Answer:
Yes
Explanation:
I believe this is a K12 question, and they want you to put "Yes" so they know you didn't slack and actually did the work.
Yes, the internal organizational consequences. The main internal organization is human resources, finance and current technology.
What are the internal and external factors affecting the business?Political, economic, societal, technical, environmental, and legal aspects are examples of external variables. These are external elements that affect the company's ability to conduct business while still having an influence. Employee morale, managerial changes, and culture shifts are examples of internal influences.
Desires, likes, dislikes, personal beliefs, and perceptions of social norms are examples of internal influences. Community members, family, culture and customs, friends, technology, and the media are just a few examples of external influences.
Poor management is one of the most prevalent internal causes of business failure. Poor employee management and poor finance management are examples of this.
Thus, Yes, the internal organizational consequences.
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Why do you believe people break laws? In your opinion what do you
believe is the main reason people end up in jail? Do you believe that jail is the best consequence? Why or why not?
Answer:
People break laws because they lack structure or are deranged.. The main reason people end up in jail could be they were having a bad day. Maybe they didn't have food on the table or needed to pay a debt off. I think jail to some is the only way to pay your crime. However, for other offenses their should be rehabilitation programs that serve a greater purpose for an inmate.
Explanation:
Suppose the principal of your public school required all students to recite a prayer at the start of each school day. What level of government (federal, state, or local) would most likely determine whether or not the principal's action was constitutional?
Answer:
Federal government is what i believe but it could be state
Federal government is the level of government would most likely determine whether the principal's action was constitutional.
What is the concept of the passage?According to the hypothetical situation in the question, the federal government would probably decide whether or not the principal's action was legal.
The municipal or state government shouldn't be in charge of this because it is a national concern. It should be mentioned that the United States Supreme Court outlawed school-sponsored prayer in public schools in 1962 on the grounds that it infringed the First Amendment.
Later, the court decided that as long as it is done discreetly and no one is coerced, students may gather and then pray on the premises of their respective schools.
In light of this, the federal authorities would probably decide whether the principal's action was legal.
Thus, it is Federal government
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