describe 2 ways of salting out proteins ​

Answers

Answer 1

The phenomenon known as "salting-out" occurs at very high ionic strengths, when protein solubility declines as ionic strength rises. As a result, salting out may be used to segregate proteins according to how soluble they are in salt solutions.

Because large levels of sodium chloride disturb the bonds and structure of the active site, the rate of enzyme activity will gradually decrease as the concentration of sodium chloride rises. As a result, some of the active sites get denaturized and the starch loses its ability to attach to them. As more enzymes get denatured and eventually cease to function, enzyme activity will steadily wane.

Describe 2 Ways Of Salting Out Proteins

Related Questions

Which molecule is polar, based on structure?
A. sulfur dioxide
OB. carbon disulphide
OC. ethylene
O D. carbon tetrachloride
Reset

Answers

Answer:

Sulfur dioxide (SO2)

Why is this the best option?

To determine the polarity of the molecule, there are two requirements. The first one has a variety of terminal atoms, whereas the second has a lone pair on the center atoms. The molecule is polar if one of the requirements is met; else, it is nonpolar. The oxygen atom is a terminal atom because it surrounds the sulfur atom, which is the center element in the SO2 molecule's Lewis structure. In this molecule, there are two terminal oxygen atoms. If we look at the first criterion, we can see that it is not met because it calls for the presence of two separate terminal atoms.

#SPJ2


What are oil traps?

Answers

Answer:

A device in which oil can be trapped, or which makes use of oil to trap something else I think

Compared to infrared radiation, an electromagnetic wave that has a lower frequency will also have a ________.

longer wavelength and lower speed
longer wavelength and equal speed
shorter wavelength and lower speed
shorter wavelength and equal speed

Answers

Compared to infrared radiation, an electromagnetic wave that has a lower frequency will also have a longer wavelength and equal speed

What is Electromagnetic wave ?

Electromagnetic waves or EM waves are waves that are created as a result of vibrations between an electric field and a magnetic field.

It can be also defined as waves are composed of oscillating magnetic and electric fields.

One important feature fo electromagnetic radiation is that all have the same speed in vacuum, c, in vacuum, which is the speed of light.

Since, the speed, wavelength and frequency of the waves are related you can infere any of them when the other two are known.

The equation is:

speed = frequency × wavelength

   s     =          ν        ×      λ

   c     =          ν        ×      λ      . . . for the specific case of electromagnetic radiation

That inverse relation between frequency and wavelength, where the speed is the constant, shows that the lower the frequency the longer the wavelength.

Therefore,  the correct statment is that an an electromagnetic wave that has a lower frequency will also have a longer wavelength and equal speed (option B).

Learn more about Electromagnetic wave here ;

https://brainly.com/question/3101711

#SPJ1

Question 3:

The force of gravity between a planet and its moon is 371 N. If the planet has a mass of 4 x 1022 kg and the moon has a mass of 5 x 105 kg, what is the distance between their centers?

Answers

Answer:

=6×10^7metre

Explanation:

By using formula,

F=GMm/d^2

Identify if the following substances (a-h) are elements or compounds. ​

Answers

Answer:

Boron, Hydrogen, Helium, Lithium, Beryllium, Carbon, Nitrogen, Oxygen, etc.

Explanation:

These are all elements, not all of them.

NH3 is produced by the reaction of 0.450 moles of N2 and 0.600 moles of H2 according to the following equation: 2N2 + 3H2 ⟶ 2NH3. (a) How many moles of NH3 are produced? (b) What is the percent yield if 5.52g of NH3 is produced?

Answers

The moles of nitrogen produced is 0.40 moles of ammonia.

The Percent yield of ammonia is 81.2%

What moles of ammonia is produced?

The moles of ammonia produced is obtained from the mole ratio of the reaction.

The mole ratio of hydrogen to nitrogen is 3 : 2

Based on the mole ratio, the limiting reactant is hydrogen.

Moles of nitrogen produced = 0.600 * 2/3 = 0.40 moles of ammonia.

mass of ammonia expected = 0.4 * 17 g = 6.8 g

Percent yield = 5.52/6.8 * 100% = 81.2%

Learn more about mole ratio at: https://brainly.com/question/19099163

#SPJ1

Magnesium burns in air with a dazzling brilliance to produce magnesium oxide: 2 Mg(s) + O2(g) → 2 MgO(s) How many moles of O2 are consumed when 2.10 mol of magnesium burns?

Answers

Answer:

1.05 mols of O2 gas

Explanation:

For this type of problem, it's important to understand what the balanced Chemical equation tells us:

Balanced Chemical equations

Let's look at the balanced chemical equation:

[tex]2Mg(s)+O_2(g)-- > 2MgO(s)[/tex]

This equation two sides of the reaction arrow.  On the left are the reactants (things you start with, and that react during the chemical reaction), and on the right side are products (things that are produced during the chemical reaction that you end with).

The numbers in front of each compound tell how many of molecules are involved for a full reaction without anything left over.  A "mol" is a large quantity ([tex]6.022*10^{23}[/tex]), in this case, of molecules , since it's unlikely you're only taking a single molecule of each substance (it would be so tiny, you wouldn't even know you were doing the reaction).

So,for every 2 moles of Magnesium used, we'll also need 1 mole of Oxygen, and it will produce 2 moles of Magnesium Oxide.

In a way, during the reaction it's almost like 2 moles of Magnesium is equal to 1 mole of Oxygen and is equal to 2 moles of Magnesium Oxide:

[tex]2 mol Mg(s)=1mol O_2(g)=2molMgO(s)[/tex]

From here, we can build some unit ratios, to convert between the known quantity of moles we have, and find the unknown quantity of moles that are requested.

Finding the right unit ratio

We know that we are looking at 2.10 mol of Magnesium, so we want a unit ratio with moles of Mg on the bottom.  We want to find moles of O2, so we want a unit ratio of moles of O2 on top.

The unit ratio we want is the middle part of the equation, divided by the left part of the equation.

[tex]2 mol Mg(s)=1mol O_2(g)\\\frac{2 mol Mg(s)}{2 mol Mg(s)}=\frac{1mol O_2(g)}{2 mol Mg(s)}\\1=\frac{1mol O_2(g)}{2 mol Mg(s)}[/tex]

Since this quantity is 1, it is a unit ratio and can be multiplied to other things to change their units (for this problem).

Finding the answer

Starting with what we know, and multiplying by our unit ratio:

[tex]2.10 mol Mg(s)*\frac{1mol O_2(g)}{2 mol Mg(s)}=1.05mol O_2(g)[/tex]

Notice that the units from the first quantity cancel with the units on the bottom of the fraction, leaving only the unit on top of the fraction ... the exact units we wanted!

So, 1.05mols of O2 would be consumed during the reaction if exactly 2.10moles of Magnesium are burned.

Chemistry: Please make sure to answer each question. Thank you in advance.


Create a diagram of your electroplating apparatus (an electrolytic cell). Then submit your drawing with the following terms labeled correctly. Or, beginning with the positive terminal of the battery and ending with the negative terminal of the battery, describe the path that the electricity takes and where each of the following occurs:

anode
cathode
copper strip
battery
positive terminal
negative terminal
place where oxidation occurs
place where reduction occurs
electrolyte solution
coin (or nail)
direction of electron flow

Answer:

(Score for Question 2: ___ of 7 points)

Describe the results of your procedure with the coin.

Answer:

(Score for Question 1: ___ of 6 points)

Write the two half-reactions that occur in this electroplating process. Be sure to include the states of the elements or ions.

Answer:

Answers

The electroplating process of the coin results in the coating of the coin with copper metal.

What is electroplating?

Electroplating is the process of using coating the surface of a metal with another one electrolytic cell.

The electroplating of the coin is done using copper as the anode and the coin as the cathode. The electrolyte is copper sulfate solution.

Oxidation occurs at the anode with the copper metal being converted to copper (ii) ions.

Reduction occurred at the cathode as the copper (ii) ions in the solution accept electrons to become copper metal and the coin being coated with copper.

The half-reactions are shown below:

At the anode:

[tex]Cu(s) \rightarrow Cu^{2+}(aq) + 2e^{-}[/tex]

At the cathode:

[tex]Cu^{2+}(aq) + 2e{-} \rightarrow Cu(s)[/tex]

Therefore, the electroplating process of the coin results in the coating of the coin with copper metal.

Learn more about electroplating at: https://brainly.com/question/3913850

#SPJ1

How many valence electrons does Rb (rubidium) have?

Answers

Answer:

The total number of electrons in a valence shell is called valence electrons. The electron configuration of rubidium shows that the last shell of rubidium has an electron. Therefore, the valence electrons of rubidium(Rb) are one.

Part E
From the observations of the simulation, which strategy was most effective for quickly and efficiently producing ammonia? Why do you think this strategy is most effective?

Answers

The production of ammonia will be favored at high pressure and low temperature.

What is an exothermic reaction?

The term exothermic reaction has to do with a reaction in which the forward reaction is favored at lower temperatures.

Now looking at the reaction coordinate and the equation of the reaction, we know that the production of ammonia will be favored at high pressure and low temperature.

Learn more about ammonia:https://brainly.com/question/12276882

#SPJ1

Identify the branch of chemistry being described.
a. _______________ study of the behavior and changes in matter including energy transfers, reaction mechanisms, and reaction rates.
b. _______________ study of compounds and chemicals containing carbon, which include pharmaceuticals, drugs, and plastics.
c. _______________ study of the components and composition of matter, including food nutrients, and quality control.
d. _______________ study of matter and processes that occur in biological organisms, including metabolism and fermentation.

Answers

Answer:

a. Physical Chemistry

b.Organic Chemistry

c.Food analysis, a very important branch of analytical chemistry

d. Biochemistry

Explanation:

Which regulates the flow of electrical current to the filament of the x-ray tube? a. high-voltage circuit
b. low-voltage circuit
c. high-voltage transformer
d. low-voltage transformer

Answers

B) low-voltage circuit

How many possible electrons can be held in the Bohr model n=8 energy level?

Answers

Electrons in the Bohr model follow the number of elements in the rows of the periodic table.

2 in the first orbit

8 in the second and third ring

18 in the fourth etc...

The Bohr model is a simplistic means of explaining the placement of the subatomic particles of the elements of the periodic table.

The Bohr model arrangement of electrons follows the same pattern as the periodic table.

2 elements in the first row, and 2 electrons in the first orbit.

8 elements in the second row, and 8 electrons in the second orbit.

8 elements in the third row, and 8 electrons in the third orbit.

The fourth and fifth orbits would therefore hold 18 electrons.

For most chemists, the Bohr model does not work well beyond the third row of the periodic table because the model becomes quite oversized and bulky.

What is the mass (in ng) of 1.23 moles of nickel?

Answers

Answer:

7.22 x 10¹⁰ ng Ni

Explanation:

To find the mass of nickel, you need to (1) convert moles to grams (via atomic mass from the periodic table) and then (2) convert grams to nanograms. These ratios need to be multiplied by each other, starting with the given value. It is important to arrange the conversions/ratios in a way that allows for the cancellation of units (the desired unit should be in the numerator). The final answer should have 3 sig figs to reflect the given value (1.23).

Atomic Mass (Ni): 58.693 g/mol

1 gram = 1 x 10⁹ nanograms

1.23 moles Ni             58.693 g               1 x 10⁹ ng
----------------------  x  ----------------------  x  ---------------------  =  7.22 x 10¹⁰ ng Ni
                                     1 mole                       1 g

Write and balance the equation for the combustion of the fatty acid lauric acid, (C12H24O2).

Answers

C12H24O2 +17 O2-------->12CO2 + 12H2O

Combustion:-

combustion is a chemical reaction that often involves the presence of oxygen and produces heat and light in the form of flames.

Lauric acid:-

Lauric acid has a 12-carbon backbone and is a saturated medium-chain fatty acid. In addition to being a key component of coconut oil and palm kernel oil, lauric acid occurs naturally in a variety of plant and animal fats and oils.

White solid lauric acid has a little bay oil odour to it.

Lauric acid is a cheap, non-toxic, and easy-to-handle substance that is frequently employed in lab studies on melting-point depression. Because lauric acid is a solid at ambient temperature but a liquid at boiling temperatures, it can be used to test different solutes to determine their molecular weights.

To learn more about Fatty acids refer to :-

https://brainly.com/question/26353151

#SPJ10

What type of reaction is represented by the following equation?

Mg(OH)2 (aq) + 2 HCl (aq) --> MgCl2 (aq) + 2 H2O (l)

Answers

Answer: Double replacement reaction

Explanation:

What is the frequency of the green light emitted by a hydrogen atom with a wavelength of 525 nm?

Answers

The frequency of the green light emitted by a hydrogen atom with a wavelength of 525 nm is [tex]5.71[/tex]×[tex]10^{14} s^{-1}[/tex]

What is wavelength?

It is the length between two successive crests or troughs of any wave.

What is a frequency?

It is the total number of waves that pass through a fixed point in unit time.

Wavelength=525nm

The relation between wavelength and frequency

[tex]v=\frac{c}{lambda}[/tex]

[tex]v=[/tex]frequency

[tex]c=[/tex]speed of light = [tex]3.0[/tex]×[tex]10^{8}[/tex][tex]m/sec[/tex]

λ(lambda)=wavelength

substitute the value in the equation mentioned above

[tex]v=3.0[/tex]×[tex]10^{8}[/tex][tex]/525[/tex]×[tex]10^{-9}[/tex]

[tex]=5.71[/tex]×[tex]10^{14}[/tex][tex]s^{-1}[/tex]

hence, the frequency of the green light emitted by a hydrogen atom with a wavelength of 525 nm is [tex]5.71[/tex]×[tex]10^{14} s^{-1}[/tex]

learn more about hydrogen emission here:

https://brainly.com/question/27231030

#SPJ10

Please and thank you

Answers

3.8mL of 0.42 phosphoric acid is required.

Reaction

2H3PO4 + 3CaCL3 → Ca3(PO)4 + 6HCl

moles CaCl2 =0.16 mol/L x0.010 L = 0.0016 mol

moles of H3Po4

= 0.0016mol of CaCl2 x 2 mole of H3PO4/3mole of CaCl2

= 0.00106 mol

V of H3PO4 = 0.0016/0.42 = 0.0038L = 3.8mL

V of H3PO4=3.8mL

To know more about calculation in milliliters refer to:-

https://brainly.com/question/23276655

#SPJ10

Solve for x , where M is molar and s is seconds.
Enter the answer. Include units. Use the exponent key above the answer box to indicate any exponent on your units.
x=(3.7×103M−2s−1)(0.37M)3

Answers

Answer:

187.416 M/s

Explanation:

First the units    M^-2 s^-1   *  M^3   =  M/s

now the numbers

3700 * .37^3 = 187.416

What can help engineers develop ways to prevent rusting?
OA. Understanding the solubility of metal ions
B. Understanding double-displacement reactions
C. Understanding oxidation-reduction reactions
D. Understanding the phase changes of metals

Answers

Answer: Understanding oxidation-reduction reactions

Explanation:

Rusting occurs when electrons are exchanged between iron and oxygen.

10. How many grams of CoCl₂ in 0.5 liters would be used to make a 1.0 molar solution? I
a. Predict the outcome if 0.5 liters of water is added to 0.5 liters of 0.70M solution of CuSO4
b. Describe what could happen is there is evaporation in the original 0.5 liters of 0.70M
solution of CuSO4

Answers

The weight of CoCl₂ used to make 0.5 ltr 1 Molar solution is 49.5g.

How to Calculate weight of substance  ?

The weight of a substance can be calculated by multiplying the number of moles of the substance by its molar mass.

However,

the number of moles of CoCl₂ must be calculated using the following formula:

no of moles = 0.5L × 1M

no of moles = 0.5moles

Molar mass of CoCl₂ = 99g/mol

mass of CoCl₂  = 0.5 × 99

mass of CoCl₂  = 49.5g

Hence, the weight of CoCl₂ used to make 0.5 ltr 1 Molar solution is 49.5g.

Learn more about Mass here ;

brainly.com/question/26416088

#SPJ1

Which of these reactions are acid-base reactions?

Answers

Answer:

The fifth answer choice (NH3 + H2O = NH4+ + OH-)

Explanation:

An acid-base reaction occurs when an acid and base combine, and the acid, (which is H2O in this case) "donates" a proton, or an H+ to the base. In this reaction, H2O gave one of its H+ to NH3, to make NH4+ and OH-. NH4+ now has a positive charge because it gained a positive ion, while OH- now has a negative charge because it gained a negative ion.

balance the chemical
reaction by providing the
correct coefficient

Answers

Answer:

2HBr + Ba(OH)2 ____ BaBr2 + 2H2O

The numbers placed in front of formulas to balance equations

How many stereoisomers could exist for the following structure?

4
8
16
32

Answers

Answer:

64

Explanation:

ieijkjkjjsjsjsjsjsjskskakskskskskkkdksksjdjdjdjdjdjdjdjdndndndmdndndmdmdndndndjdjdjdjdjdkdkekrk

calculate the molecular weight of carbon dioxide

Answers

Answer:

44.01 g/mol

Explanation:

Carbon dioxide is produced when carbon and oxygen are burned.

To totally burn carbon into carbon dioxide, or CO2, we require one mole of oxygen for every gram of carbon by weight.

or alternatively:

You produce 12+216=44 g of carbon dioxide for every 12 g of carbon.

The weight of CO2 in kg may be calculated if the amount of CO2 in litres is known by converting it to moles and multiplying it by 44.

5 Examples of simple salts

Answers

Five Examples of Salts

Sodium Chloride. Sodium chloride (NaCl) is the most common type of salt in our lives. Potassium Dichromate. •Potassium dichromate (K2Cr2O7) is an orange-colored salt composed of potassium, chromium and oxygen. Calcium Chloride. Sodium Bisulfate. Copper Sulfate.

pls tell this answers ​

Answers

Answer:

okay

Explanation:

9 . acidic

10.  hydrogen

answer the following questions

11. Gold as a precious metal is used widely in making jewelry items. This metal is preferred over others for jewelry pieces for various reasons. One important reason for its popularity is that it does not react with atmospheric moisture and therefore, it does not rust.

12. corrosion is defined as rust it's due to humidity and moisture

13. Sulphur appears much below chlorine in the reactivity table and cannot displace chlorine from dilute HCl.

14. Highly conductive metal

Surpassed only by silver, copper is a highly conductive metal. This means electricity can pass through it with greater ease, making it ideal for use in electrical wires. Companies can use other conductive metals to create electrical wires.

15. Corrosion

Copper metal when exposed to air turns green in color due to corrosion. When the copper vessel is exposed to air in the rainy season, the metal reacts with gases, moisture, and atmospheric gases to form a mixture of copper carbonate and copper hydroxide. This gives a green color to the surface of the copper meta

16. Silicon

silicon is the backbone of the electronics industry. Because silicon is the reason for the fast development happened in the field of electronics. therefore, silicon is the backbone of electronics.

17. High chemical reactivity

Sodium is stored under kerosene because of its high chemical reactivity with almost all of the other elements (for example oxygen from the air which oxidizes it instantly). Kerosene and mineral oil are the only chemicals that make sodium inert. Usually, when Na reacts with a solvent it liberates hydrogen gas from its composition.

18. Metal oxides are oxides of metals. Nonmetal oxides are oxides of nonmetals. The main difference between metal oxides and nonmetal oxides is that metal oxides are basic compounds whereas nonmetal oxides are acidic compounds.

19.  Silver is more likely to tarnish near a petroleum refinery than in a place far away from it because when silver reacts with petroleum or hydrogen sulfide present in it as a pollutant in the air, it starts forming a black coating of silver sulfide.

20. A noble metal is ordinarily regarded as a metallic chemical element that is generally resistant to corrosion and is usually found in nature in its raw form. Gold, platinum, and the other platinum group metals (ruthenium, rhodium, palladium, osmium, iridium) are most often so classified. Silver, copper, and mercury are less often to sometimes included as noble metals although each of these usually occurs in nature combined with sulfur.

A 10g of limestone is treated with dilute hydrochloric acid.The liberated gas measured at 27°C and 750mmHg is 18.5dm^3.Find the percentage purity of calcium carbonate.​

Answers

Your question's data is not correct, it should be 100 grams of limestone instead of 10 grams, You will find the correct solution below according to that.

The percentage purity of calcium carbonate is 82.5%

The balanced chemical equation is

CaCO₃ + 2HCl  →  CaCl₂ + H₂O + CO₂

Because CO₂ is a gas, you can easily convert it to mole directly

No. of mol of CO₂ = volume dm³ /22.4 dm³

No. of mol of CO₂ = 8.5 dm³ / 22.4 dm³

No. of mol of CO₂ = 0.825 mol

According to equation 1 mole of CO2 is made from 1 mole of CaCO3

So, 0.825 mole of CO2 will be made from 0.825 mole of CaCO3

Mass of CaCO3 = moles of CaCO3 × Molar mass of CaCO3

Mass of CaCO3 = 0.825 × 100 grams

Mass of CaCO3 = 82.5 grams

Percentage purity of CaCO3 = (mass of CaCO3/Total mass of CaCO3) × 100

Percentage purity of CaCO3 = (82.5/100) × 100 %

Percentage purity of CaCO3 = 82.5 %

Thus we can say that as percentage purity is the percentage of mass of pure compound on per unit of total compound, the percentage purity of the calcium carbonate will be 82.5 %

Learn more about Percentage Purity here:

https://brainly.com/question/25446369

#SPJ10

draw a diagram to show how you will separate the salt from sugar using the solvent ethanol

Answers

i can’t draw sorry but tbh i’m just trying to get sum points so i can do my work u feel me ;)

Which of the following methods correctly describes the preparation of 1.00 L of an aqueous solution of 0.500 M NaOH?

Answers

Answer:

Place 20.0 g NaOH(s) in a flask and dilute to 1.00 L with water.

Explanation:

Other Questions
the price of an mp3 player is increased by 8%to 52.92what was the original price? Researchers designing studies to be conducted in host countries outside the u. S. Should: PLEASE HELP ILL GIVE BRAINLIEST PLEASE PLEASE PLEEEEEEEAAAAASEWrite the recursive formula for the arithmetic sequence.A(n)=(-1)+(n-1)(-5) A snowboarder on a slope starts from rest and reaches a speed of 4.2 m/s after 7.3 s Select the correct answer.Consider function f.f(x) = 2xWhat is the value of ()(x)O A.8x4OB. 16x4OC. 4x44x2D. In my country, we make so much waste-we buy and throw away, buy and throw away, buy and throw away-and yet northern countries will not share with the needy. Even when we have more than enough, we are afraid to share, we are afraid to let go of some of our wealth.The Girl Who Silenced the World for Five Minutes,Severn Cullis-SuzukiBased on the passage, why are the people in Suzukis country wasteful?because they do not have enoughbecause they are afraid of people in the southern countriesbecause they share too much with othersbecause they are afraid to lose wealth What two houses of Congress were created by the Great Compromise? Symptoms of malaria appears when In a train timetable, in order to estimatearrival and departure time, read thetimes from Which word is the best antonym for invincible. a. often victoriousb. quickly forgottenc. easily defeatedd. not athletic Want to earn 20 points for correct answer CLICK ME A firm operating in a perfectly competitive market is a price taker because: If side b measures 53, what is the length of side c? Development refers to the pattern of continuity and change in human capabilities that occurs _____. A medical assistant is answering the clinic phone. Which of the following guidelines demonstrates professional telephone etiquette? 50 people were asked if they speak French or German or Spanish. Of these people, 31 speak French 2 speak French, German and Spanish 4 speak French and Spanish but not German 7 speak German and Spanish 8 do not speak any of the languages all 10 people who speak German speak at least one other language How many people only speak Spanish? Hassan put 437.2 grams of brown sugar into a canister. Then he carefully measured out 430.43 grams of it to use in a recipe for cookies. How much brown sugar is left in the canister? What error did Lucia make? What is the median of the data? Plato trigonometry is crazy