Electroporation is a technique used for __________

Answers

Answer 1

Electroporation is a technique used for inserting DNA into cells

What is Electroporation?

Electroporation is a physical transfection method that uses an electrical pulse to create temporary pores in cell membranes through which substances like nucleic acids can pass into cells.

Electroporation is highly efficient for the introduction of foreign genes into tissue culture cells, especially mammalian cells.

Hence, electroporation is a technique used for inserting DNA into cells

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Related Questions


1. When mRNA moves out of the nucleus into the cytoplasm it attachs to another RNA
called rRNA, a protein assembly site referred to

Answers

mRNA moves out of the nucleus into the cytoplasm and attaches to another RNA called rRNA, a protein assembly site referred to as the ribosome.

What is Ribosome?

This is referred to an organelle which is responsible for protein synthesis in cells.

The mRNA is used as a template for the rRNA to attach at this point to aid in protein translation and production. rRNA ensures that the appropriate amino acids are added and also form part of the ribosome which are involved in catalysis of the reaction.

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Two different bacterial samples, a and b, were inoculated onto sim media. What can you conclude based on your observations?

Answers

The conclusion that can be made here is that:

A is motileSpecimen A gave indoleSpecimen B does not hydrogen sulfide (H2S)Specimen B does not have indole

What is a sim media inoculation?

This is a type of laboratory practical that isb used to see if organisms are motile through the use of single stabs.

An organism is motile when there is a radiation from the stab mark which makes the tube it is in to turn turbid.

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in analyzing the number of different bases in a DNA sample, which result would be consistent with the base-pairing rules?

Answers

In analyzing the number of different bases in a DNA sample, the result would be consistent with the base-pairing rules is A+G = C+T.

What is the RNA base pairing rule?

Base pairing occurs precisely: a purine base binds to a pyrimidine – adenine (A) on one chain pairs with thymine (T) on the other and guanine (G) pairs with cytosine (C).

In this case, the base pairing rule states that the number of adenine is equal to number of thymine. Similarly, the number of guanine is equal to number of cytosine.

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Why do calculations of recombination frequencies between loci that are far apart on chromosomes underestimate the true genetic distance between the loci?.

Answers

This is the complete question

A. Recombination frequencies cannot exceed 50% and asymptotically approach 50% as the distance between genes increases because only one crossover can occur per chromosome. B. As the distance between loci increases, some multiple crossovers go undetected such that the relationship between recombination frequency and map distance ceases to be linear. C.Loci that are far apart on a chromosome often recombine to produce combinations of alleles that result in a lethal phenotype and such recombinants are not recovered or identified. D. Loci that are far apart on a chromosome are often susceptible to a form of chromatic interference that results in reduced crossing over, and this interference does not occur for loci that are closer together. E. If two crossovers occur between two loci, then only nonrecombinant gametes are produced; therefore, recombination frequencies actually begin to decrease for more distant pairs of genes.

As the distance between loci increases, some multiple crossovers go undetected such that the relationship between recombination frequency and map distance ceases to be linear.  Thus, option "B" is correct.

How, explain your answer briefly?

When calculating recombination frequencies, and hence, map distances, we might notice that these distances are not completely additive. They might vary. For example, let us say that we have three genes, A, B, and C, in that order. We calculated that the distance between A and B equals 5.9 MU and that B and C are 19.5 MU apart. According to this, we might say that the total distance between A and C is 25.4 MU (5.9 + 19.5). However, after a two-point calculation between A and C, the value equals 23.7 MU.

The recombination frequency between these two genes located in the extremes and far apart underestimates the actual genetic distances between them because there might occur other crossing-overs that were not detected. This is when calculating the distance between A and C, we probably will not detect the occurrence of a double recombinant between them, and hence, we might sub-estimate the real distance.

The relationship between the actual map distance (number of crossing overs) and the recombination frequency between two loci, is not lineal. The farther apart are the two genes, the worse is the distance estimation.

Thus, option "B" is correct.

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Control group is important for scientific study ? and why ? ​

Answers

An unaffected group is always important to have so we can compare the results of the study to something that hasn’t been affected by whatever the study is doing.

What environmental impacts does green roofs and double window glazing have?

Answers

Answer:

A green roof can increase building efficiency and help reduce electricity costs.

Explanation:

I hope this helps!

which part of the body contains enyzmes that break down protein

Answers

Answer: Stomach

Explanation:

The stomach produces pepsin which is an enzyme that digest proteins.

Answer:

In the stomach, pepsin is the main digestive enzyme attacking proteins.

An organ is a part of the body composed of two or more tissue types and is designed to perform at least one specific physiological function

Answers

An organ is a body part that is made up of two or more different tissue types and is intended to carry out at least one particular physiological function. An organ system is a collection of organs that cooperate to meet the body's essential needs and provide for it.

describe 4 differences between a bactrial cell and a plant cell

Answers

Answer:

Genetic material, cell wall, photosynthesis, and ribosomes.

Extras: vacuole, flagella

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Anemia is a medical condition in which the affected person lacks enough red blood cells. What two effects will such a condition have on the patient?

Answers

The two effects will such a condition have on the patient are as follows:

The patient will have less hemoglobin in the blood.The patient will have a reduced oxygen supply to the cells.

What is Anemia?

Anemia may be defined as a condition that leads to a deficiency of hemoglobin or Red blood cells in the blood.

The complete question is as follows:

The patient will have less hemoglobin in the blood.The patient will have less immunity against bacteria.The patient will have a reduced oxygen supply to the cells.The patient will have a thicker blood composition.The patient will have increased platelets in the body.

Red blood cells are accountable for carrying oxygen to cells and when a patient is suffering from anemia it leads to a reduced oxygen supply throughout the body.

Therefore, the correct options are A and C.

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Can you think of a physiologic process that is not under the control of a homeostatic reflex system?.

Answers

Hair and nail growth are not homeostatically controlled. There is no miracle in using exuberance to handle these procedures. The disadvantage is that they can produce very long and interrupt normal activities.

Hair and nail growth are not homeostatic ally controlled.  The disadvantage is that they can produce very long and interrupt normal activities.

What is homeostasis ?

It is a physiological process where body to maintain the internal environment in response to changes in outer external environment.

Internal environment means the interstitial fluids while external environment refers to the environment in which organisms live.

Any  fluctuation in homeostasis leads to death or a disease of an organism, called as homeostatic imbalance.

The diseases include diabetes, dehydration, hypoglycemia, hyperglycemia, gout etc.

The controlling factors of homeostasis are stimulus can be any physical, chemical or environmental factors  leads to deviation of normal body’s environment

Receptor receives the stimulus and pass to the control center like neuron.

Regulator can control coordinator center receives and processes information from the receptor like Hypothalamus.

Effector can control commands from stimulus like Glands, muscles

Response refers to any reaction response to the stimulus.

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how many nanometers is the best resolution achieved with using a light microscope?

Answers

The best resolution that can be achieved with light microscopes is; 200nm

What is the resolution of the microscope?

The light microscope is also called optical microscope and it is a type of microscope that primarily uses visible light and a system of lenses to produce  magnified images of smaller objects.

The best resolution that can be achieved with light microscopes is about 0.2 microns or 200nm because the maximum useful magnification it can provide is approximately 1,000x.

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An immunocompromised host is one who shows a decrease in ______.

Answers

An immunocompromised host is one who shows a decrease in the ability to mount an immune defense to infection because the immune system is damaged and it is weak which can not fight infections.

What is immunocompromised host?

An immunocompromised host refer to a person whose immune system is weak or damage and does not have tye ability to fight incoming infections.

Therefore, An immunocompromised host is one who shows a decrease in the ability to mount an immune defense to infection because the immune system is damaged and it is weak which can not fight infections.

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In a scientific investigation, the term “quantitative data” refers to numbers that are usually followed by
units.
graphs.
written descriptions.
photos of measurement.

Answers

Quantitative data in scientific investigations are numbers usually followed by units. The first option is the correct one.

What are quantitative data?

In relation to scientific investigation, quantitative data are attributes that are measurable or quantifiable. Thus, they can be assigned numerical values.

Because of the measurability, variables within quantitative data usually have units attached to them.

For example, height is measured in meters, feet, centimeters, etc.

Quantitative data is opposed to qualitative data because the latter cannot be assigned a numerical value.

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Answer:

A. units.

Explanation:

i got it right

The chart below shows the three main types of plant tissues and associated tissues.

The chart shows the 3 main types of plant tissues and associated tissues. Plant tissue is divided into dermal, ground, and 1. Ground is divided into 2, Collenchyma, 3. 1 is divided into 4 and 5.

Which row (A, B, C, or D) best completes the chart above?


1
2
3
4
5
A
Vascular
Xylem
Parenchyma
Phloem
Sclerenchyma
B
Xylem
Phloem
Vascular
Sclerenchyma
Parenchyma
C
Parenchyma
Vascular
Xylem
Phloem
Sclerenchyma
D
Vascular
Sclerenchyma
Parenchyma
Xylem
Phloem
A
B
C
D

Answers

The plant tissues can be subdivided in the chart as Vascular Sclerenchyma Parenchyma Xylem Phloem. Thus, the correct option is D.

What role does the sclerenchyma play?

Sclerenchyma's primary job is to offer mechanical support. They are made up of lengthy, slender cells that have a thick lignified wall. They lack protoplast and are largely dead.

The complete chart and table are given in the image attached.

A basic permanent tissue known as parenchyma makes up a large portion of the ground tissues of plants, where vascular tissues and other tissues are attached. They are non-vascular and made up of undifferentiated, primitive living cells that have undergone modifications to carry out distinct tasks.

Xylem, along with phloem and cambium, is a key component of vascular tissue. Its primary functions include providing mechanical support and storage as well as moving water and solutes from the plant-soil interface to the stems and leaves.

Parenchyma cells, are also a component of phloem and are essential for the storage of water, non-structural carbohydrates, and storage proteins.

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Answer: it’s D

Explanation: truss

The process during which a cell eats itself is referred to as.

Answers

Autophagy I believe that the answer you are looking for is autophagy

What does the author predict about the future of the Earth's mantle? Do you support his educated guess? Use complete sentences to answer.

Answers

Because this region is immune to human meddling, the earth's mantle won't change in the future.

What transpires in the mantle of the Earth?

We can determine the Earth's topography thanks to the heat and material transport taking place in the mantle. Plate tectonics is shifted by mantle activity, which also causes earthquakes, volcanoes, seafloor spreading, and the formation of mountains.

Therefore, since this area is protected from human interference, we can draw the conclusion that the earth's mantle won't alter in the future.

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which part of the microscope reflects light

Answers

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which part of the microscope reflects light?

THE ANSWER IS MIRRORS

Why mirrors? bcs mirrors are sometimes used in lieu of a built-in light. If your microscope has a mirror, it is used to reflect light from an external light source up through the bottom of the stage. Nosepiece: This circular structure is where the different objective lenses are screwed in.

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correct me if I’m wrong

1. What are the independent and dependent variables in each of the experiments?

2. What information should be added to the diagram to give the reader a better understanding of how these experiments
were conducted?

3. What items should have been CONTROLLED in the experiments?

4. How much confidence would you have in the conclusion of experiment 3 if you found out that the temperature was not
controlled? Explain your reasoning.

5. Create your own flow chart to answer a causal question.

Answers

The independent variables are temperature, airflow, and light and the dependent variable in each of the experiments is evaporation.The schematic representation in the diagram helps in the understanding of the setup for the experiments.The level of temperature, the rate of airflow, and the intensity of light must be controlled in the experiments.A person will not be confident in experiment 3 results as the temperature is the independent variable that alters the results of evaporation.

What are the four foremost factors of a managed test?

When possible, scientists take a look at their hypotheses through the use of managed experiments. A managed test is a systematic take look at executed below-managed conditions, which means that simply one (or a few) elements are modified at a time, even as all others are saved consistently. We'll appear carefully at managed experiments within side the subsequent section.

In the three experiments, the dependent variable is evaporation, as it changes with the change in another variable.

The independent variable in experiment 1 is temperature, experiment 2 is airflow, and experiment 3 is light.

The experiment can be understood better with the representation of the schematic that shows the setup to analyze how the experiment was performed. Therefore the diagram must add the schematic of the experiment.

The controlled items in the experiment are the variables that are set at the particular unit to form the result. the controlled item in experiment 1 is temperature, experiment 2 is airflow from the fan, and experiment 3 is the intensity of light.

The result of experiment 3 is based on the dependence on the intensity of light keeping temperature constant and controlled. If the temperature is not controlled in the experiment then it may lead to an alteration in the results as it depends on both light and temperature.

The flow chart for the increase in pressure affecting the evaporation is given in the image attached.

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from about 450 to 300 million years ago, ______ dominated earth's vegetation, and then about 300 million years ago, ______ began to become more prominent.

Answers

From about 450 to 300 million years ago, Bryophytes and seedless vascular plants dominated Earth's vegetation, and then about 300 million years ago, Gymnosperms began to become more prominent.

From about 450 to 300 million years ago, bryophytes dominated the earth's vegetation, and then about 300 million years ago, gymnosperms began to become more prominent.

Evolution of plants

About 450 to 300 million years ago, most of the plants as we currently have them are yet to evolve according to evolutionists.

Instead, seedless vascular plants such as bryophytes were around. Many years after, these seedless plants evolved into vascular plants with seeds that are not enclosed in fruits.

Vascular plants with seeds but without fruits are known as gymnosperms. Plants whose seeds are enclosed in fruits, angiosperms, evolved thereafter.

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A bird's wing and the wing of an insect are examples of

Answers

A birds wing and the wing of an Insect are examples of; analogous organs

What are the organs of the animal?

A birds wing and the wing of an Insect are examples of analogous organs.

Analogous organs are the opposite of homologous organs and they are defined as  are those organs of different animals that look different in structure but perform a common function.

An example of an analogous trait is the wings of insects and birds that evolved independently in each lineage separately after diverging from an ancestor without wings.

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An organ system is a group of organs that work together to perform _____ functions in a multicellular organism

Answers

Answer:

a certain functions

Explanation:

How does dermal tissue help plants on a hot day?
It releases substances so plants can absorb more water from the soil.
It has a waxy material that prevents the loss of water to the environment.
It has guard cells that open to prevent the loss of water to the environment.
It contains chemical substances that allow plants to use water more quickly.

Answers

It has a waxy material that prevents the loss of water to the environment.
It has a waxy material that prevents loss of water to the environment.

_______ uses a rigid or flexible scope, which is inserted through the urethra and advanced into the renal pelvis and ureter.

Answers

The blank space in the task content should be filled with; Ureteroscopy.

What is a Ureteroscopy?

The ureteroscopy examination, involves inserting a thin, flexible scope into the patients bladder and ureter (the urine-carrying tube that carries urine from the kidneys to the bladder). This is done in a bid to detect abnormalities such as kidney stones.

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What does integrin do?

Answers

Integrin acts as transmembrane linkers and mediates the interactions between the cytoskeleton and the extracellular matrix.

What is Integrin?

They are proteins which possesses catalytic and structural functions in the body of organisms.

This is done through their ability to regulate different reactions which occur in cells.

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Arrange the following events in the order in which they occurred.

Answers

Answer:

1→4→5→3→2

Mitotic cell division starts with the condensing of chromosome accompanied by splitting of the centrosome and its movement towards the opposite poles.

As the nuclear envelope and nucleolus disappear, spindle fibres arise from the centrosomes and start binding to the condensed chromosomes at the centromeres.

The chromosomes bound by the spindle fibers are then arranged in the middle of the cell forming the equatorial metaphase plate.

Next the centromere splits and the sister chromatids are pulled away towards the poles by the contraction of spindle fibres.

Following this, a cleavage furrow starts to develop in the middle of the cell while the nuclear membrane starts to reappear around the separated chromatids.

The condensed chromatids start thinning into chromatin fibre along with reappearance of the nucleolus and the cleavage furrow deepening further.

Finally as the nuclear membrane becomes fully intact, the cleavage furrow deepens and split the cells from middle into two daughter cells.

Consider the diagram of an internal feedback mechanism that the body uses to help maintain homeostasis.

The internal feedback mechanism is shown. Stimuli goes to the receptor to the coordinator to the effector to the response and then to feedback.

What is the role of the central nervous system in this model?

a) coordinator
b) effector
c) response
d) stimuli

Answers

The role of the central nervous system in this model is as coordinator (Option a).

What is the central nervous system?

The central nervous system is a fundamental body system that acts by sending and receiving signals from the environment.

The central nervous system can act as a coordinator by modulating signals and thus maintaining the internal equilibrium (homeostasis).

In conclusion, the role of the central nervous system in this model is as coordinator (Option a).

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Regarding the factors of true labor: distortion of the myometrium increases the sensitivity of the skeletal muscle layers. the fetal pituitary releases oxytocin in response to estrogens. placental estrogens decrease the sensitivity of the muscle cells of the myometrium. maternal oxytocin release is inhibited by high estrogen levels.

Answers

Regarding the factors of true labor, the the fetal pituitary releases oxytocin in response to estrogens.

What is oxytocin?

It should be noted that oxytocin means a hormone that is involved in childbirth. It is associated with trust and empathy.

In this case, regarding the the factors of true labor, the the fetal pituitary releases oxytocin in response to estrogens.

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In which situation would new technology be most likely to cause a change in an existing theory about new structures a cell?
The new technology makes it possible to detect and analyze a structure more rapidly.
The new technology allows scientists to confirm the presence of an existing structure in a cell.
The new technology makes it possible to produce additional structures that were not previously there.
The new technology allows detection of a structure that could not be detected previously.

Answers

The situation that the new technology be most likely to cause a change in an existing theory about new structures of a cell is the new technology allows detection of a structure that could not be detected previously.

What is a technology?

Technology is the application of scientific knowledge for practical purposes. Technology is used in the advancement of the worlds. New technologies help to solve many problems that were impossible before.

Thus, the correct option is D.

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The new technology allows detection of a structure that could not be detected previously. Thus, the correct option is D.

What are the benefits of technology?

Sales will probably improve as a result of technology that makes it simpler for people to buy from you, including an e-commerce solution and numerous payment apps. Marketing departments are forced to adapt the social media platforms that your clients and potential customers use since they are born and die on a monthly basis.

The tasks that HR departments perform for hiring, reviewing applicants, onboarding, and training involve a significant amount of technology. The more HR duties you can complete using technology, the quicker they can be completed and the less likely it is that you will make a mistake that could result in a lost recruit, an employee leaving, or a lawsuit. Employee education on topics like benefit enrollment and corporate policies, for instance, guarantees appropriate paperwork is done and cuts down on training time for new hires.

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why do the potato strip in pure water get bigger?​

Answers

Answer:

The incoming water in the potato cells pushes on the cell walls and makes the cells bigger , that's how the strips of potato in pure water becomes bigger .

This is because of osmosis, water from outside the potato strip travels down the concentration gradient and into the potato strip causing it to get bigger.
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Phase change involves changing states of matter (solid-liquid-gas). How do these three states of matter differ from each other? (on a molecular level) Hello hello :))) please help me with this problem Please reply please help1. Give two reasons why people came to America from other countries.2. List four countries from which people came to America in the 1800s.3. How was the government of the U.S. different from that in many of the immigrants' home countries?4. How did the Homestead Act affect immigration into the U.S.?a) It attracted more immigrants with the offer of free land.b) It caused immigration to slow down because all land was already taken by citizens.c) It stopped newcomers from signing labor contracts before they came in.d) It restricted Asian people from coming in.5. California became a state after people gathered there from all over the world becausea) Poor people there could get free food and housing.b) It was the only place where foreigners were welcomed.c) Gold had been discovered.d) Wages for foreigners were higher than in other places.6. A person becomes an immigrant when he/shea) Follows a gold rushb) Leaves his/her home and comes to a new country to livec) Travels a long wayd) Visits a new country7. Imagine you live somewhere in Europe in 1890. Your family has lived in the same place for generations. What factors in your homeland might cause you to decide to leave your home and move to America, as millions did at that time?8. As a European of 1890, you hear much about the newest nation in the world America. What are you hearing that might make you decide to leave your home and move to the U.S.? Both armies had an unusual __________, however.a. overconfidenceb. ability to recover quickly Seventy years ago, an advanced cataract ___________ doomed the sufferer to creeping blindness.a. selectivelyb. statisticallyd. invariably Piet Mondrians work used: A. dull colors.B. naturalistic colors.C. only red, blue, and yellow. D. vivid, wild colors. What is the sentence pattern of the sentence my cousins were in the cafeteria A 210-N mass is suspended from a horizontal beam by two cables that make angles of 29 degrees and 53 degrees with the beam. Find, using vectors, the tension in the cables. Find the surface area of a hexagonal prism if the length of each side of the hexagonal base is 4 cm and the height is 7 cm. Write an explicit rule to represent the sequence:20, 24, 28, 32, 36,...a) f(n)=4n+20b) f(n)=4n+16c) f(n)=4n+24d) f(n)=4n+12 60 ml of a 1.0 m solution of h2so4 is mixed with distilled water to make 1 l of solution. what is the molarity -Find the area of a rectangle with a length of 15 inches and a width of 9 inches. The area of the rectangle isinches. Enter only the number.The solution issquare Which of the following is TRUE about the solar oven?1. A solar oven converts the Sun's light energy to heat energy.2. A solar oven is inexpensive since it is built from low-tech device.3. A solar oven is environment-friendly, very easy to use, cheap, and safe.4.All of the choices. Arms reduction and settling border disputes contributed to the league of nations goal of. Which equation justifies why 93 - ?=(0) 1_9(+)_993()93_9(+)=9(0) -9393=9=9_g(-)_9=9=9 Raphael and Martina are engaged and are planning to travel to Las Vegas during the 2020 Christmas season and get married around the end of the year. In 2020, Raphael expects to earn $45,400 and Martina expects to earn $15,800. Their employers have deducted the appropriate amount of withholding from their paychecks throughout the year. Neither Raphael nor Martina has any itemized deductions. They are trying to decide whether they should get married on December 31, 2020, or on January 1, 2021. What do you recommend? Use the appropriate Tax Tables. the table below reports quarterly gdp and real gdp data for the united states during the great recession, which lasted from the end of 2007 to mid-2009. What did the Townshend Duties do? They required that all legal documents and newspapers be printed on special watermarked paper. They set moderate tax rates on certain imported items to the colonies. They imposed such heavy duties on imported goods that colonists could no longer afford to buy them. They removed taxes on all items except those being shipped to the British West Indies. They raised large amounts of revenue and helped to reduce the British treasury's serious deficit. be found3.Change the sentences to the structure: "have something done" or "getsomething done." (12 points)1- We edited the article. (have)2- William made the necklace. (get)3- I delivered the furniture to the factory. (have)4- The mechanic has fixed the brakes. (have) a(3.75)=6.11What is a?