if an uncut plasmid travels much faster through a gel than expected, but still only gives one band, what could be the potential explanation

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Answer 1

Because the plasmid supercoiled, and therefore was able to move through the gel much more quickly than expected, but still only gives one band.

There are two types of uncut plasmids: relaxed and supercoiled (or superhelical). When compared to a rubber band, these two shapes are easier to comprehend. The relaxed form is comparable to a regular rubber band. The band will coil into a compact shape resembling a supercoiled plasmid if you hold one side of the band between your thumb and forefinger and roll that side. The band won't continue to twist if you cut it with scissors.

Supercoiled plasmids may pass across a gel significantly more quickly than a linear DNA fragment with the same amount of base pairs because of their small size. Similar to how sliced fragments of the same mass normally move more slowly than uncut relaxed plasmids do.

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Related Questions

Which answer choice lists the steps of the scientific method in the correct order? a. observation, hypothesis, experiment, conclusion, scientific theory b. scientific theory, conclusion, hypothesis, experiment, observation
c. hypothesis, observation, experiment, conclusion, scientific theory d. observation, experiment, hypothesis, conclusion, scientific theory e. conclusion, hypothesis, observation, experiment, scientific theory

Answers

The formulation of scientific theories supported by experimental data will be the last phase. The current alternative, which involves observation, hypothesis, experiment result, and then science, is option one.

The first step in the scientific technique of problem-solvingsolving Mcq is which of the following?

The "Question" is the first phase of the scientific method. The "Problem" is another name for this step. You should formulate your query in a way that allows experimentation to provide a solution. So that everyone understands the problem you are attempting to tackle, keep your inquiry succinct and straightforward.

What does the scientific approach resolve?

Making conjectures (hypothetical explanations), drawing predictions from the hypothesis as logical consequences, and finally conducting experiments are all steps in the scientific method.

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a cell is said to be in which state when it has an equal movement of molecules into and out of cell

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The cell is said to be at an equilibrium state when it has an equal movement of molecules into and out of the cell.

At equilibrium, the movement of motes continues, but because there's no attention grade, there's no net movement in any particular direction. However, its patches will tend to move toward the area where it's less concentrated until equilibrium is reached If the substance can cross the cell membrane.

At that point, the attention of the substance on both sides of the cell membrane will be the same. Prolixity is the movement of any substance from an area of high attention into an area of low attention.

Ultimately, the solute will fully disconnect from its concentrated form and spread out unevenly within the detergent. At this point, the solute has reached equilibrium within the detergent.

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_____ aid in the coordination of the activities of adjacent animal cells.a). Plasmodesmatab). Keratin fibersc). Tight junctionse). Desmosomesf). Gap (communicating) junctionsExpert Answer

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Gap (communicating) junctions help in the coordination of the actions of adjacent animal cells.

Gap junctions can be considered as leaky junctions that connect the two corresponding cells in animals and therefore establish connection between them. The cytoplasm of the two cells remain in direct contact due to gap junctions.

Animal cells are the type of eukaryotic cells that do not contain the cell wall. They consist of plasma membrane and all of the cell organelles like mitochondria, nucleus, endoplasmic reticulum, lysosomes, golgi body, etc. However, animal cells do not possess the chloroplasts as present in plant cells. They contain centrioles specific to animal cells only.

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What neurotransmitter will result in constriction of the pupil? a. norepinephrine b. acetylcholine c. epinephrine d. serotonin

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d)serotonin. Pupil constriction is caused by the neurotransmitter acetylcholine.

In the brain and throughout the body, serotonin is a neurotransmitter that communicates between nerve cells. Mood, sleep, digestion, nausea, wound healing, bone health, blood coagulation, and sexual desire are just a few of the bodily processes that serotonin is essential for.How do you feel when you take serotonin?

Serotonin in the brain is thought to control emotions like happiness and worry. Increasing serotonin activity in the brain is thought to be how selective serotonin reuptake inhibitor (SSRI) drugs work to treat mood disorders.

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a woman homozygous for the gene mutation that causes cystic fibrosis, with a partner who does not carry the same mutation, has what chance of having a child with the disease?

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Answer:

A woman who is homozygous for the gene mutation that causes cystic fibrosis has a 25% chance of having a child with the disease if her partner does not carry the same mutation. This is because the mutation is recessive, so the child must inherit two copies of the mutated gene in order to display the trait. If the woman's partner does not carry the mutated gene, then the child only has a 25% chance of inheriting two copies of the mutated gene and thus displaying the trait.

how are sebaceous glands associated with hair follicles

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Typically linked to hair follicles, sebaceous glands secrete a fatty substance called sebum into the follicular duct, which subsequently travels to the skin's surface.

With the exception of the palms of the hands and the soles of the feet, the glands are found all over the body, although they are most prevalent on the scalp and face.The sebaceous gland releases sebum through the sebaceous duct that connects the gland to the hair follicle, which is a combination of fats (triglycerides, wax esters, squalene, and cholesterol) and cellular waste. Sebum contributes to the formation of the skin's thin, somewhat oily surface coating, keeping the skin flexible and preventing it from absorbing too much water.During the fourth month of pregnancy, sebaceous glands start to emerge from embryonic hair follicles, and by the time a baby is born, they are enormous and fully formed.

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where is mitosis in the cell cycle before and after?

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Mitosis is a stage in the cell cycle that follows the interphase and is followed by the interphase again.

Before mitosis, the cell is in the interphase which is composed of three stages: G1, S, and G2. In G1 stage, the cell carries out normal metabolic processes, in S stage DNA is replicated, and in G2 stage the cell checks for any damage to the replicated DNA before mitosis.

After mitosis, the cell enters into the interphase again, where it goes through the same three stages (G1, S, and G2) before entering mitosis again. This is the process that results in the formation of two identical daughter cells with the same number of chromosomes.

Some cells like nerve cells and muscle cells, will enter a different stage called G0, which means they are not actively cycling and will not divide again.

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A cell has 16 chromosomes. after mitosis, how many chromosomes will the daughter cells have?

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If a cell with 16 chromosomes goes through mitosis, each daughter cell will have 16 chromosomes as well.

Mitosis is a cell division process that produces two identical daughter cells.

Because DNA was replicated prior to mitosis, these daughter cells have the same genetic material.

Mitosis is a nuclear division process that occurs in eukaryotic cells when a parent cell divides to produce two identical daughter cells. Mitosis is the separation of the duplicated genetic material carried in the nucleus during cell division. Prophase, prometaphase, metaphase, anaphase, and telophase are the conventional stages of mitosis. There is no cell growth during mitosis, and all cellular energy is directed toward cell division.

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Which of these energy sources is increasing its relative share of the global energy supply by the most since 2015?a. renewables b. nuclear c. coal d. oil

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Renewables (a) have been increasing their relative share of the global energy supply the most since 2015.

What are renewables?

Renewables are energy sources that are replenished naturally and do not deplete finite resources. They are considered to be sustainable and environmentally friendly alternatives to non-renewable energy sources such as coal, oil, and natural gas.

The use of renewable energy sources, such as wind and solar power, has been growing rapidly in recent years due to a combination of declining costs, technological advancements, and increasing concern about climate change.

According to the International Energy Agency (IEA), the share of renewables in global power generation increased from 24% in 2015 to 30% in 2020. During the same period, the use of coal (c) decreased from 38% to 27%, while the use of oil (d) remained relatively stable at around 25%. The share of nuclear energy (b) also remained relatively stable, hovering around 5-6%.

The trend towards increased use of renewables is expected to continue in the coming years, with many countries setting targets to transition to 100% renewable energy in the coming decades.

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Rank from the first to the last steps to describe the correct order of events to maintain homeostasis.

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The steps necessary to maintain sodium ion homeostasis have been taken. Outside of cells, sodium ion concentration is regulated by a negative feedback loop. See explanation for further information.

When there is a little amount of extracellular sodium ions, the macula densa's receptors identify them and the RAAS is initially engaged. Renin is therefore released, activating aldosterone and angiotensin-II in the process. Angiotensin-II and aldosterone reabsorb sodium ions in the nephron tubules. Aldosterone and angiotensin-II release are reduced, water absorption increases, and the sodium ion level returns to normal. A chemical element with the atomic number 11 and the letter Na is sodium (derived from the Latin natrium). It is an extremely reactive, delicate silvery-white metal. Sodium is an alkali metal since it is in group 1 of the periodic chart.

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Which statements are true about mechanical barriers? (1) Mechanical barriers physically block pathogens from entering the body. (2) The skin is the most important mechanical barrier. (3) Mechanical barriers are living organisms that help protect the body. (4) Mechanical barriers destroy pathogens on the outer body surface.

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Mechanical barriers physically block pathogens from entering the body. The correct option is A.

What are mechanical barriers?

The first line of defence against pathogens is mechanical barriers, which physically prevent pathogens from entering the body.

The skin, mucous membranes, and bodily fluids like tears and urine serve as mechanical barriers that prevent infections from entering the body.

Enzymes in saliva, perspiration, and semen act as chemical barriers that eliminate infections on the surface of the body.

The skin and the tissue layers that face the lung's airways are also referred to as epithelia and serve as the body's external biological barriers, much as the surface of the intestines.

Thus, the correct option is A.

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Why does meiosis produce cells with fewer chromosomes?

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Answer is meiosis is sexual reproduction that joins two cells that each have half the number of chromosomes.

Explanation: gametes have half the total number of chromosomes as somatic cells because when a sperm fertilizes an egg, their chromosomes will unite to make a full set. In the process of fertilization, the sperm fuses with the egg cell and they become one cell.

Does breast tenderness come and go early pregnancy?

Answers

Yes, breast tenderness can come and go in early pregnancy.

The soreness can be constant, or it can come and go. During the earliest weeks of pregnancy, breast pain tends to be dull and achy. The breasts may feel heavy and swollen. They can be super sensitive to the touch, making exercise and sex play very uncomfortable.

Just like other early pregnancy symptoms, breast tenderness can come and go. Some women are lucky and don't have much trouble with early pregnancy symptoms at all. Other women experience pregnancy symptoms right up until the time they give birth. Both circumstances are completely normal.  It may even continue through the latter part of the gestation for some women.  

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In a food web, green plants take in carbon dioxide and water to make sugars in the presence of sunlight.

How are carbon dioxide and water returned to the enviroment?

A. by decomposing the dead parts
B. by becoming food for the herbivores
C. by transpiration and decomposition
D. by cellular respiration and transpiration

Answers

Answer:

C. by transpiration and decomposition.

Explanation:

A. Green plants return carbon dioxide to the environment through a process called photosynthesis, in which they take in carbon dioxide and water, and using the energy from sunlight, convert it into glucose, a type of sugar. They also give off oxygen as a byproduct.

B. When herbivores consume the plants, they take in the carbon and energy stored in the plant's tissues, but the carbon is returned to the environment through the herbivore's own metabolism and excretion.

C. Transpiration is the process by which water is lost through the leaves of the plant, and it is returned to the environment through evaporation. Decomposition is the process by which dead plant material is broken down by microorganisms, releasing the stored carbon and other nutrients back into the environment.

D. Cellular respiration is the process that green plants and all other living organisms use to release energy stored in glucose, by converting it into ATP, a molecule that stores energy. Transpiration is the process by which water is lost through the leaves of the plant, and it is returned to the environment through evaporation.

how long does it take for a broken collarbone to heal

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While it is possible for a broken collarbone to take longer, the typical recovery time for people is between 6 and 8 weeks. Usually, it takes 3 to 6 weeks for children to recover from an injury. To get your shoulder back to full strength, though, it will take at least that long again.

Broken collarbones are a regular accident. The collarbone, also called the clavicle, joins the breastbone to the shoulder blade. Sports, falls, and auto accidents are common causes of broken collarbones. While giving birth, babies can break their collarbones.

If you have a broken collarbone, get medical attention right away. Most injuries recover quickly with ice, painkillers, a sling, physical therapy, and time. For some breaks, surgery may be necessary to insert plates, screws, or rods into the bone to keep the fragments together while the  bone heals.

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why do some animals migrate While others are able to live in the same place year around

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Because basically stay in the same places for awhile but they move often as well

3) which statement about the complement system is true? a) these proteins are involved in innate immunity and not acquired immunity. b) these proteins are secreted by cytotoxic t cells and other cd8 cells. c) this group of proteins includes interferons and interleukins. d) these proteins are one group of antimicrobial proteins acting together in cascade fashion. e) these proteins act individually to attack and lyse microbes.

Answers

Option D that is these proteins are one group of antimicrobial proteins acting together in cascade fashion about the complement system is true.

What is antimicrobial proteins?

Antimicrobial peptides and proteins (AMPs) are a varied family of naturally occurring chemicals generated by all multicellular organisms as a first line of defense. These proteins have the potential to destroy bacteria, yeasts, fungus, viruses, and potentially cancer cells. Human host defense antimicrobial peptides and proteins (AMPs) serve an important role in warding off invading microbial infections as crucial components of innate immunity. AMPs can also perform various biological roles such apoptosis, wound healing, and immunological regulation. Antimicrobial proteins and peptides (AMPs) are ancient components of host defense that help neutrophils destroy intraphagosomes and are produced into extracellular fluids by epithelial cells at mucosal surfaces and in the skin (Wiesner and Vilcinskas, 2010).

Here,

Option D that is True, these proteins are one set of antimicrobial proteins that work in a cascade way on the complement system.

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the part of the respiratory system that is the voice box

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larynx is the part of the respiratory system that is the voice box. it is made of Membranes, which also help hold cartilage together.

The larynx is a component of the respiratory system. It is a hollow tube that transports air from your throat (pharynx) to your trachea and then to your lungs. It also houses your vocal cords and is necessary for human speech, so it is also known as the voice box. Your larynx is located in the center of your neck, near the Adam's apple. It's between the fourth and sixth cervical vertebrae (neck bones). The larynx serves three primary functions in the body: Breathing. Making vocalizations. Keeping food and other particles out of your trachea, lungs, and other respiratory organs.

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Which of the following groups of cellular components are found in eukaryotic cells but not prokaryotic cells?An endoplasmic reticulum, mitochondria, and a nucleus

Answers

Answer:

the endoplasmic reticulum, mitochondria, and a nucleus are cellular components that are found in eukaryotic cells but not prokaryotic cells. means all the choice are correct

Explanation:

Organelles called mitochondria are present in eukaryotic cells. They are in charge of creating the ATP, which the cell uses for a variety of metabolic functions. In addition to breaking down lipids and proteins, mitochondria also contribute to the production of hormones and neurotransmitters.

Eukaryotic cells have a membrane-bound organelle called the nucleus. It houses the chromosome-organized genetic material of the cell. The control of the cell's functions, such as growth, metabolism, and reproduction, rests with the nucleus.

f this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? quizzlet

Answers

Answer:

By the sperm cell dividing or by 5 sperm cells enter the egg at the same time

Explanation:

One sperm cell can break down into two to or two sperm cell reach the egg at the same time resulting in either Identical twins (monozygotic) or Fraternal (dizygotic) so that theory explain in Quintuplets

the hip bone joins the end of the thighbone. select one: a. proximal b. distal c. lateral d. medial

Answers

The upper (proximal) end of the femur connects to the hip joint. So, the correct option is (A).

What is Hip joint?

The hip joint is defined as a ball-and-socket joint that allows motion and gives the stability needed to bear the weight of the body where the socket area called the acetabulum is inside the pelvis and The ball part of this joint is above the thighbone which is called femur. It attaches to the acetabulum to form the hip joint.

The function of hip joint is that the hip acts as a multi-axial, ball-and-socket joint on which the upper body is balanced during stance and gait, where the balance and stability provided by the hip joint allow for motion. while supporting the forces encountered during daily activities.

Thus, the upper (proximal) end of the femur connects to the hip joint. So, the correct option is (A).

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true/false. Lysosomes digest food particles brought into a cell by endocytosis. After a vesicle containing food particles fuses with a lysosome, H ions are transported into the lysosome from the cytosol. This significantly lowers the pH of the lysosome relative to the cytosol and activates the enzymes that digest the particles.

Answers

Answer:

True. Lysosomes are membrane-bound organelles that contain digestive enzymes that can break down large molecules, such as proteins, carbohydrates, and lipids. When a vesicle containing food particles fuses with a lysosome, the pH of the lysosome is lowered relative to the cytosol by the transport of H ions into the lysosome from the cytosol. This lower pH activates the enzymes that digest the particles.

natural selection can be summarized by which statement? group of answer choices within a population, some variations are favored by environmental conditions and others are not none of the answers are satisfactory all species are fixed organisms transform and pass these transformations on to their offspring

Answers

The correct statement about natural selection is People who possess the most advantageous traits live and procreate.

A population's members are naturally variable, which means that they are all distinctive in some ways.

This variation indicates that some people have characteristics that are more environment-appropriate than others.

The process by which "organisms with more advantageous traits" are more likely to survive and procreate is known as "natural selection." The process of "differential survival" and "reproduction in organisms" with different phenotypes is known as natural selection. The struggle for existence, variation, and inheritance is represented by the conditions highlighted in bold above. According to some, these circumstances are both necessary and sufficient for natural selection to take place.

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Which factor is not required for natural selection to occur? variation within a population inheritance of traits selective pressure an anticipated change in the environment genetic differences

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Natural selection is the process by which organisms that possess certain traits are more likely to survive and reproduce than those without the traits.

This process is responsible for the gradual evolution of species and is one of the most important mechanisms of evolutionary change. In order for natural selection to occur, several key factors must be present, including variation within a population, inheritance of traits, selective pressure, and genetic differences.

The factor that is not required for natural selection to occur is an anticipated change in the environment. While environmental changes can certainly influence the process of natural selection, they are not a necessary component. For example, if a population has sufficient variation and is subject to strong selective pressures, natural selection can still occur even if the environment remains constant. Therefore, while environmental change can be an important factor in the process of natural selection, it is not a requirement for natural selection to occur.

In conclusion, for natural selection to occur, several key factors must be present, including variation within a population, inheritance of traits, selective pressure, and genetic differences. An anticipated change in the environment is not required for natural selection to occur, although it can certainly influence the process. By understanding the components of natural selection, we can gain a better appreciation for the process by which species evolve and adapt to their environment.

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the spotted lanternfly is a herbivorous insect that is native to asia and feeds on over 70 species of plants. the feeding behavior of the spotted lanternfly damages plants, and the nymph and adult lanternflies secrete sticky residues that exacerbate the damage to native plant species by promoting mold growth. the spotted lanternfly reproduces once during its lifetime by laying between 30 and 50 eggs, and the eggs hatch after a short amount of time. the range of the spotted lanternfly is increasing because the insects are transported to new environments by the movements of animals and people, and it is now found in many parts of the united states. which of the following best describes the spotted lanternfly in the united states? responses the spotted lanternfly is a k-selected and invasive species. the spotted lanternfly is a k -selected and invasive species. the spotted lanternfly is a k-selected and endangered species. the spotted lanternfly is a k -selected and endangered species. the spotted lanternfly is an r-selected and invasive species. the spotted lanternfly is an r -selected and invasive species. the spotted lanternfly is an r-selected and endangered species.

Answers

The correct response to the question is that spotted lanternfly is K selected and invasive species.

It is possible to infer that the spotted lanternfly is invasive and K-selected from the description.

Organisms are classified as being r-selected or K-selected based on the trade-off between quantity and quality of offspring. Compared to r-selected species, K-selected species have fewer progeny.

We can infer that lanternflies are K-selected because they only reproduce once during their lifetime.

Additionally, the spotted lanternfly is invasive because of the harm they do to local plant species, which causes those species to become endangered.

Consequently, the spotted lanternfly is invasive and K-selected.

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living cells have evolved mechanisms that upregulate the production of atp in response to increased levels of atp hydrolysis; based on information in this passage, why has the ability to hold atp concentration far above equilibrium concentration in cells provided a selective advantage?

Answers

In response to higher levels of ATP hydrolysis, living cells have mechanisms that have evolved that upregulate the synthesis of ATP. Given the information in this section, why has the ability to maintain ATP concentrations well above concentration in cells offered a selection advantage.

reduces these cells' G'o for Hydrolysis of atp

reduces the ATP hydrolysis Keq in these cells

increases these cells' ATP hydrolysis Keq

reduces these cells' G for Adenosine triphosphate

What exactly is a balance?

The state of equilibrium is one in which the forward and backward rates of response are equal.

How does hydrolysis work?

In a typical type of chemical reaction called hydrolysis, the chemical properties that already exist are primarily broken down by water.

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the endocrine system has many functions including ______.

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The Endocrine System has many functions including -Controlling movement of food through the digestive tract,Regulating blood ions concentrations,Maintaining blood volume.

The body is covered with a network of glands and organs known as the endocrine system. It functions crucially in regulating and controlling various bodily processes, just like the nervous system does.The endocrine system, on the other hand, employs chemical messengers known as hormones while the neurological system communicates through nerve impulses and neurotransmitters.By releasing hormones, the endocrine system is in charge of controlling a variety of body processes.The endocrine system's glands release hormones, which then circulate through the blood to the body's organs and tissues. These organs and tissues are subsequently given instructions by the hormones on how to behave or perform.

complete question:The Endocrine System has many functions including ____________. (select all that apply)

a) Producing neurotransmitters

b) Controlling movement of food through the digestive tract

c) Regulating blood ions concentrations

d) Maintaining blood volume

e) Receiving sensory information from the environment

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how do the two members of a pair of homologous chromosomes differ from each other?

Answers

Two members of a pair of homologous chromosomes differ from each other because of the different alleles.  

The chromosomes of a homologous pair each have the same genes in the same order, but differences between them can result in different alleles. Some of these allelic variants have functional implications and others do not. Her two members of a homologous chromosome are different from each other because they have different versions of the same gene called alleles. As humans, our somatic cells are diploid. That is, there are two copies of each chromosome. We get one copy of each chromosome from our parents, one of hers from our mother, and one from our father.

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help me pleasee i need thiss

Answers

Answer:

The chromosomes in the homologous pair each have the same genes in the same order, but there might be variations between them, resulting in different alleles.

Explanation:

suppose you compared the nucleotide sequences of the distal control elements in the enhancers of three genes that are expressed only in muscle cells. what would you expect to find? why?

Answers

We would expect to find that the regulation elements of the enhancers for the three genes should share some sequence similarities or be identical.

Why would you expect to find mentioned above in respect to asked statement?Due to this commonality, the enhancers of all three genes may bind to the same distinct transcription factors, which would then coordinately boost their expression.What do you mean by Gene Enhancers?

Basically the Gene enhancers are DNA-regulatory components that increase the transcription of a gene or genes more than would occur without them. By generating chromatin loops, these components work distantly to bring the enhancer and target gene close together.

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How far away can the moth be detected by the bat, so that the echo from one chirp returns before the next chirp is emitted? you arrive on scene to find a conscious 58-year-old woman sitting up and reporting severe chest pain and shortness of breath. she is anxious and tells you she feels like she is going to die. physical examination shows that her skin is pale, cool, and clammy and her pulse is rapid, weak, and irregular. her breathing is labored, with a respiratory rate of 28 breaths/min. her spo2 is 90%. lung sounds show crackles in all fields. blood pressure is 92/60 mm hg. what is your differential diagnosis of this patient? ANALYZING KEY IDEASAND DETAILS1. DETERMINING CENTRALIDEAS What are two possibleoutcomes when each house ofCongress has to agree on thewording of a bill in order topass it?2. CITING TEXT EVIDENCEWhat information from the textsupports the idea that therewas concern about the amountof time elected representativeshad to pass laws after losingreelection? What principles did the framers establish in the Constitution? 6. Mila operates a backhoe and needs to know how tall it is.Draw6 ft52130a. Find two corresponding sides. Use them to create a scale factor ratio. Include units! (1)b. Use your ratio to set up equivalent fractions and find the height of the backhoe. (2) Tom makes $ in 281.10 days. How much does he make in 5 days? which answer best explains why marketing organizations continue to advertise even when those marketers realize that most targeted consumers are no longer paying attention to the ads? reflect on your process or performance on this multiple-choice practice. choose one of the following questions to answer in a written response of approximately 150-200 words. make sure you answer the question thoroughly and provide detail from the questions and/or the passages as necessary. if you used the technique of dissecting the answer choices and then eliminating, was this process helpful? do you think you answered more questions correctly as a result, or did the process help you move through the quiz more quickly? explain why or why not. describe your process. if you used the technique of dissecting the answer choices and then eliminating, were there any words or terms used in the answer choices that made it difficult for you to eliminate? if so, did you find a way around this challenge? describe your process. choose one question you think you might have missed. describe what was challenging about the question or answer choices. which answer did you end up choosing? what led you to conclude it was the best answer? describe your process in arriving at your answer, including any test-taking techniques you used. What is the term used for the variable that receives an argument that passed into a module?A ) GlobalB ) LocalC ) ParameterD ) ConstantE ) None of these HELPPPPPPPPPPPPPPPPPPPPPP