the denaturing solution contains the detergent sodium dodecyl sulfate (sds) and the reducing agent 2-mercaptoethanol. why is it necessary to use these compounds in this procedure? what do they do to prepare the proteins for running a page gel?

Answers

Answer 1

Sodium dodecyl-sulfate polymeric agarose gel (SDS-PAGE) is frequently used to separate complicated protein mixtures at high resolution. The peptides that will be subjected to electrophoresis are first denatured by the technique.

An electrophoresis is what?

A laboratory procedure called electrophoresis is used to divide DNA, RNA, or protein molecules according to their size and electrostatic current. The molecules are moved by an electric current thru a gel or other medium.

RNA: what is it?

All living cells contain ribonucleic acid, a nucleic acid that resembles DNA in structure.

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Related Questions

explain how a rechargeable battery can be used as a model of adp and atp

Answers

The ATP molecule is just like a rechargeable battery. When it's fully charged, it's ATP. When it's run down, it's ADP.

However, the battery doesn't get thrown away when it's run down–it just gets charged up again. All biological organisms contain the crucial chemical ATP (adenosine triphosphate). Consider it the cell's "energy currency." The ATP molecule splits off one of its three phosphates when a cell has to expend energy to complete a task, resulting in the formation of ADP (Adenosine di-phosphate) + phosphate. That phosphate molecule's energy is no longer bound to it and can now be used by the cell to perform tasks. The cell stores extra energy by reattaching a free phosphate molecule to ADP, converting it back into ATP. This extra energy can come from the breakdown of food that has been ingested or, in the case of plants, energy produced during photosynthesis.

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what would be the advantage of using a molecular model that shows the compounds involved during cellular respiration to compare the amounts of carbon released into the atmosphere?(1 point) responses allows the comparison between cellular respiration and photosynthesis in the movement of carbon allows the comparison between cellular respiration and photosynthesis in the movement of carbon allows for the prediction of certain environmental conditions that could affect the process allows for the prediction of certain environmental conditions that could affect the process allows a visual of the process that is not visible to the human eye allows a visual of the process that is not visible to the human eye allows a comprehensive view of the entire carbon cycle allows a comprehensive view of the entire carbon cycle

Answers

We can visualise chemical molecules that are invisible to the human eye thanks to molecular models.

Three-dimensional visual representations of molecules are known as molecular models. We can more clearly visualise a molecule's appearance thanks to molecular models. We can gain a better understanding of the potential changes that could take place in a molecule by using a molecular model. The science of numerically representing molecular structures and simulating their behaviour using quantum and classical physics equations is known as molecular modelling.

The benefit of using a molecular model to compare the amount of carbon released into the atmosphere and the compounds involved in cellular respiration is that it provides a visual of the process that is invisible to the human eye.

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Recall the experiment on ant navigation in which investigators were testing the hypothesis that ants were able to count the number of steps between their nest and a food source. To run a controlled experiment, what parameters were held constant for the test groups of ants

Answers

All variables except leg length , The ant navigation demonstrates the different movements of the ants. The ants generally navigate based on the sun's rays and the availability of the food in a particular direction.

Scientists can perform the different experiments to find navigation in ants. Different parameters and objects can be used for the identification of the ants navigation.  The length of the ants must be keep constant and all other variables can be changed. Thus, the correct answer is all variables except leg length.

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1. each branching point represents a point in evolutionary history where an ancestral evolutionary lineage gave rise to a new lineage. the most recent common ancestor shared by all lineages in this cladogram is represented by the number fill in the blank text field 1 enter... . the most recent common ancestor shared only by gymnosperms and angiosperms is represented by fill in the blank text field 2 enter...

Answers

Each branch point in an evolutionary tree symbolises a time in the past when an ancestor lineage gave rise to a different lineage. A cladogram is a visual representation of the creatures and different species that have ancestors with similar lineages.

A branch point designates the location where one species is different from another. The precise moment at which two evolutionary lineages diverged from a single ancestor is called a branch point. Frequency-dependent selection can cause evolutionary branching by dividing a population with a monomorphic phenotype into two different phenotypic clusters. The population must be driven by directional selection toward a fitness minimum in phenotype space in order for evolutionary branching to occur. A divergence event is represented by each branch point, also known as an internal node.

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which of the following statements is false? a. organisms placed in the same genus are least closely related. b. the scientific name for humans is homo sapiens. c. moving from species to kingdom, more different species are included in each higher category. d. species that are in the same genus share very specific characteristics. e. taxonomists are biologists who classify organisms.

Answers

False statements is a. organisms placed in the same genus are least closely related.

In general, Genus is termed as a group of related species which has more features related to species of other genera. Hence ,Organisms that are placed in the same genus are very closely related.

So, Two species present within the same genus will share a recent common ancestor in their evolutionary history. These two species will be closely related to each other than two species classified into different families. Therefore, members representing  the same genus, but different species interbreed, could produce infertile offspring. example include mule, which is the product of a cross between a male donkey and a female horse.

Hence, A is correct option

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When comparing complete tetanus with unfused tetanus, which is true?A) In complete tetanus, no relaxation occurs between stimuli.B) In complete tetanus, maximum tension is developed.C) In complete tetanus, the muscle fiber is stimulated at a higher frequency.D) A and BE) A, B, and C

Answers

The true statement when comparing  complete tetanus with unfused tetanus is: (B) In complete tetanus, maximum tension is developed.

Tetanus is a disease of the muscles caused to a bacterium called Clostridium tetani. The bacterium releases harmful toxins in the body that causes painful contractions. In severe conditions, the disease can even lead to death.

Tension in the muscles is the physiological condition where they remain in semi-contracted form for long durations of time. This can result in formation of lumps called muscle knots in the body. It is the situation when the myosin heads remain attached with thin filaments.

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when a muscle is held during a stretch for a prolonged period, the gto is activated, which results in the muscle relaxing. what is this action called?

Answers

when a muscle is held during a stretch for a prolonged period, the gto is activated, which results in the muscle relaxing. this action is called as  autogenic inhibition.

When the Golgi tendon organ (GTO) and muscle spindles are activated, certain muscles are inhibited from contracting. These two musculotendinous proprioceptors, which are found in and around joints and muscles, respond to changes in muscle tension and length, which aids in the management of muscular control and coordination.

When the muscle contracts or stretches, the GTO, which is located between the muscle belly and the tendon, detects increased tension. When a muscle contracts, the GTO is activated, and it responds by inhibiting the contraction and contracting the opposing (antagonist) muscle group. Autogenic inhibition is the name given to this process.

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D. It has long been known in the field of human genetics that wavy hair is the expression of a heterozygous genotype, while curly (C1C1) and straight (C2C2) hair is the expression of a homozygous genotype. If a man with curly hair marries a woman with wavy hair, what percentage of their children will have curly, wavy, and straight hair

Answers

Answer:

50% will have curly hair (C1C1) . 50% will will have wavy hair(C1C2). 0% would have straight hair (C2C2).

Explanation:

given the information the man has C1C1 or curly hair and the woman has wavy hair which should be C1C2. Construct a simple punnet square and solve the genotypic ratio. witch is (C1C1) = 2/4 = 50% And (C1C2) = 2/4 = 50%

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The endocrine system releases chemical messengers called , _________ into the blood stream

Answers

Answer: Hormones

Explanation:

Hormones are the chemical messengers secreted by the endocrine glands in the blood.

Answer:

hormones

Explanation:

Natalie wants to do a report about growing human organs in livestock.
She plugged five key words into a search engine: human, pig,
transplant, organ, and science. Which of the following search results
would be the most reliable sources for her report? Choose the two that
apply.
A. Front page article in the Daily Post newspaper,
"Scientists Clone
New Creature, Half-Human, Half-Pig!"

B. Science article in The Economist magazine, "Stem cells and gene
editing may turn domestic animals into organ factories."

C. Buzzfeed website, "
"Scientists just created the first Human-Pig
Embryo.

D. Video on Federation of American Scientists website, "Pigs: Source
of Replacement Organs for Humans?"

Answers

Answer:

B and D

Explanation:

A science article in an accredited magazine and a video on the Federation of American Scientists website would be the most credible two sources

A newspaper article and Buzzfeed would be less credible

While the atria are relaxing, the ventricles are in systole/diastole and the tricuspid and bicuspid valves are open/closed whereas the semilunar valves are open/closed

Answers

While the atria are in diastole (relaxing), the ventricles are in systole (contracting). The tricuspid and bicuspid valves (also known as the atrioventricular valves) are closed during ventricular systole, and the semilunar valves (the aortic and pulmonary valves) are open, allowing blood to be pumped out of the ventricles into the aorta and pulmonary artery.

While the atria are relaxing and filling with blood, the ventricles are contracting and expelling blood. This is known as the cardiac cycle, which is divided into two phases: systole and diastole.

During diastole, the atria are in a relaxed state while the ventricles are in a relaxed state. In this phase, the tricuspid and bicuspid valves, also known as the atrioventricular valves, are open, allowing blood to flow from the atria into the ventricles.

The semilunar valves, the pulmonary and aortic valves, are closed, preventing blood from flowing back into the ventricles from the pulmonary artery and aorta.

As the ventricles contract during systole, the tricuspid and bicuspid valves close, preventing blood from flowing back into the atria. This increase in pressure in the ventricles causes the semilunar valves to open, and blood is forced out of the heart and into the circulatory system. The atria then begin to fill with blood again, and the cycle repeats.

The cardiac cycle is controlled by electrical impulses generated by the sinoatrial (SA) node and the atrioventricular (AV) node, which coordinate the contraction of the atria and ventricles.

The heart's ability to pump blood efficiently is dependent on the proper timing and coordination of the cardiac cycle. Overall, this cycle is vital for the oxygenation and nutrition of the body by pumping the blood and supplying it to all the body parts.

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microscopic examination of an animal cell reveals the presence of a plasma membrane but no cell wall. which additional structures would normally be present within this cell?

Answers

Answer:

Microscopic examination of an animal cell reveals the presence of a plasma membrane, but no cell wall. Other structures that would normally be present within this cell include a nucleus, endoplasmic reticulum, Golgi apparatus, mitochondria, vacuoles, and lysosomes. Additionally, animal cells usually contain cytoskeletons, which are composed of various proteins such as microtubules and actin filaments that provide structure and support to the cell.

two linked genes(a) and (b) are seprated by 18 m.u. a man with genotype aa bb marries a woman who is aa bb. the man's father was aa and bb. what is the probability that their first two children will both be ab/ab?

Answers

The probability that their first two children will both be ab/ab genotype is (41/100) ×(41/100) =0.1681.

The genetic distance between two genes is 18 cM. This suggests that recombination frequency between two genes is 18%. Hence the recombinant gamete produced by heterozygous parent is 18%

The parental gametes are 100-18= 82%

Now it is given that man's father is AABB. This suggest that man's genotype is in cis condition. Man's genotype is AB/ab

Parents : AB/ab  (Man)  × ab/ ab (Woman) 

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Most psychiatric hospitals are a form of primary care.


True


False

Answers

Answer:

True

Explanation:

hope this helps

This is true hope this is helpful

What do the rough endoplasmic reticulum, Golgi apparatus, and lysosomes have in common? (multiple choice) They are all membranous organelles comprising the endomembrane system. They all perform similar roles in the cell. They are all physically connected to each other. All are located in the nucleus of the cell

Answers

Being membrane-bound organelles, the rough endoplasmic reticulum, Golgi apparatus, and lysosome share this trait. Organelles that are membrane-bound are portions of the cell that, like the phospholipid bilayer membrane that surrounds the entire cell, are protected by a membrane.

The endomembrane system, which generates and transports proteins throughout the cell, includes the endoplasmic reticulum and the Golgi. The lysosome functions as the cell's recycling facility, disintegrating used and unwanted materials as needed.

Eukaryotic cells contain the Golgi apparatus and endoplasmic reticulum, two crucial cell organelles. Both are composed of fluid-filled sacs surrounded by membranes. However, whereas the Golgi apparatus is not connected to the nucleus, some portions of the ER are continuous with the nuclear membrane.

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to separate organelles that are close in size, one employs the technique called sucrose gradient centrifugation. this technique separates organelles by:

Answers

to separate organelles that are close in size, one employs the technique called sucrose gradient centrifugation. this technique separates organelles by density

Cells can be broken down in a variety of ways, including osmotic shock or ultrasonic vibration, being forced through a small orifice, or being ground up in a blender. Many of the cell's membranes (including the plasma membrane and endoplasmic reticulum membranes) are broken into fragments that immediately reseal to form small closed vesicles. However, when done correctly, the disruption procedures leave organelles like nuclei, mitochondria, the Golgi apparatus, lysosomes, and peroxisomes largely intact. The cell suspension is thus reduced to a thick slurry (called a homogenate or extract) containing a variety of membrane-enclosed organelles, each with a distinct size, charge, and density.

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Defend the claim that ribosomes are the most essential organelle in the cell. In your reasoning include evidence of at least 3 organelles that support the ribosome in accomplishing its function for the cell.

Answers

Answer:

The ribosome is an essential organelle for many reasons in below

Explanation:

The ribosome is an essential organelle because it is responsible for the synthesis of proteins. Proteins are the most important components of cells, as they serve as a component of all cellular functions such as cell metabolism, replication, and communication.

The cell has three other organelles: mitochondria and endoplasmic reticulum. Both of these organelles perform important tasks within the cell, but neither of them can synthesize proteins. Therefore, it is clear that without the ribosome, there would be no way for cells to synthesize proteins or perform any other tasks.

In addition to being essential to cellular function, the ribosome has been shown to have other important functions in its own right. For example, it has been shown that the ribosome can bind DNA and repair damage caused by free radicals (a type of chemical reaction). Furthermore, some research has shown that certain diseases such as cancer can be caused by defects in ribosomal complexes or genes that encode parts of these complexes.

finger-like membrane extensions, known as microvilli, are supported by the actin cytoskeleton. microvilli located at the apical surface of epithelial cells are responsible for:

Answers

Answer:

Microvilli located at the apical surface of epithelial cells are responsible for increasing the absorptive surface area of the cell and increasing the efficiency of nutrient absorption. They are also involved in secretion of substances, as well as in recognition of antigens and other molecules. Microvilli are supported by the actin cytoskeleton, which is composed of a network of actin filaments that provides structure and stability to the microvilli.

quizlt a point mutation in the ligand binding site of a na channel will most likely a. turn a na channel into a k channel b. have no effect c. turn this ligand-gated channel into a voltage gated channel d. prevent channel opening in the presence of the ligand e. prevent channel closing

Answers

The correct option is e, that is prevent channel closing. A na channel's ligand binding site mutation will probably prevent the channel from closing.

What occurs when a ligand attaches to a Na channel that is ligand-gated?

Ligand-Gated Ion Channels

When a ligand binds to the receptor's of exofacial surface, a conformational change occurs that causes a pore to open, allowing certain ions to pass through the channel and across the plasma membrane.

The function of ligand-gated Na channels :

Neurotransmitters are bound via ligand-gated ion channels, which then open in accordance to ligand binding. Synaptic transmission among two neurons either between a neuron and a muscle is regulated by these channels.

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what is needed in the culture medium of animal cells? select all that are minimally necessary for all animal cell cultures

Answers

In the culture medium of animal cells, it required nutrients, growth factors, and amino acids that are generally not synthesized by cells, hence A, B, and C are the correct options.

What are the requirements of the animal cell culture?

If there is a need to grow animal cells in the culture medium, it requires all the necessary substances, which grow the cells in the tissue.

Glucose and amino acid are major requirements to make cells in living conditions, metabolism provides energy to the cells. Growth factors are also required to increase cell size.

Therefore, A, B, and C are the correct options.

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The given question is incomplete, so most probably complete  question is,

What is needed in the culture medium of animal cells? select all that are minimally necessary for all animal cell cultures

A. nutrients such as glucose

B. growth factors

C. any amino acids or vitamins that the cells cannot make themselves

D. bovine serum albumen

E. carbon dioxide

Which of the Following organisms Reproduce through Spore Formation?

Answers

Many different types of organisms reproduce through spore formation, including:

Fungi: Many types of fungi, such as mushrooms and mold, reproduce through the production of spores.

Algae: Many types of algae reproduce through the formation of spores.

Bryophytes: mosses, hornworts, and liverworts reproduce via spores.

Ferns: Ferns reproduce via spores.

Some bacteria: Some types of bacteria, such as Bacillus and Clostridium, reproduce through the formation of spores.

Note that spore formation is one way of reproducing, but not all organism that can form spores reproduce only via spores. Some may have other ways of reproducing as well.

Answer:

Fungi reproduce through spore formation; for e.g., bread moulds.

Explanation:

what four structure do eukaryotes and prokaryotes have in common?

Answers

The architecture of prokaryotic and eukaryotic cells are similar. DNA, cytoplasm, ribosomes, and the plasma membrane are present in every cell.

Four elements are found in every cell: the plasma membrane, which separates the interior of the cell from its surroundings; the cytoplasm, which is the cell's interior jelly-like space; the DNA, which serves as the genetic makeup of the cell; and the ribosomes.

Arrangements of phospholipids and proteins called lipid bilayers are found in both eukaryotic and prokaryotic cells, and they serve as a physical barrier between the interior and exterior environments of the cell.

However, a eukaryotic cell is often bigger than a prokaryotic cell, contains a genuine nucleus, which means that its DNA is encased by a membrane, and includes additional membrane-bound organelles that enable functional categorization.

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which of the following best explains why triploid bananas do not produce seeds? responses the cells of the banana plant are unable to replicate dnadna, thus preventing cell division and limiting growth. the cells of the banana plant are unable to replicate d n a , thus preventing cell division and limiting growth. the banana plants lack enough genetic diversity to properly hybridize. the banana plants lack enough genetic diversity to properly hybridize. the production of gametes is disrupted because of unequal pairing of homologous chromosomes during meiosis. the production of gametes is disrupted because of unequal pairing of homologous chromosomes during meiosis. the production of seeds is not required because triploid plants produce gametes without fertilization.

Answers

DNA replication is not possible in the banana plant's cells, which prevents cell division and restricts growth.

Successful seed germination is extremely rare in triploids because they rarely produce eggs or sperm with a balanced set of chromosomes.

Most sterile plants don't produce any fruits, and triploid bananas are sterile. The banana fruit, which is the mammalian equivalent of a placenta without fertilisation or an embryo, is produced without any seeds, however. Bananas are sterile and seedless, like other odd polyploids (with three sets of chromosomes), because one set of chromosomes (A or B) lacks a homologous set to pair with during synapsis of meiosis. Because of this, meiosis is disrupted, and no viable gametes (sex cells) are produced.

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describe the procedure you followed to gram-stain bacteria. what is the purpose of each of the specific reagents?

Answers

The most frequent test to determine whether you have a bacterial infection is a Gram stain. The test will indicate whether your infection is Gram-positive or Gram-negative if you do.

What are Gram positive bacteria?

If you are exhibiting signs of a bacterial illness, you could require this test. Numerous bacterial illnesses frequently exhibit the symptoms of pain, fever, and weariness.

The type of infection you have and where in your body it is located will determine any additional symptoms.

Depending on the type of illness, your doctor may need to collect a sample from the suspected infection site or from specific body fluids.

Therefore, The most frequent test to determine whether you have a bacterial infection is a Gram stain. The test will indicate whether your infection is Gram-positive or Gram-negative if you do.

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1. (03.06 LC) Match the mixture with its description. (4 points) 1. Solution 2. Emulsions 3. Homogeneous 4. Colloid a. Microscopically insoluble particles that are suspended throughout another substance b. Two or more liquids that are normally not mixable c. A solute that is distributed evenly within the solvent d. A mixture that has its different components mixed evenly within the substance

Answers

A particular homogeneous mixture in which the constituents of one or more elements are uniformly distributed throughout another substance. a mixture of two liquids, typically immiscible

What sort of mixing is an illustration of homogeneity?

A combination of sugar and water, for instance, or salt and water These mixtures are both instances of homogenous mixtures since they have the same components throughout.

The meaning of homogenous

Generally speaking, homogenous refers to having same parts or components. Homogeneous refers to something that is identical in nature of character throughout. A variety of things that are essentially similar to one another or belong to the same kind can also be referred to as homogenous.

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what are the similarities and differences between the lactase persistence mutations found in african populations and the one found in european populations

Answers

Lactase persistence mutations observed in African and European populations are both point mutations that appear in distinct sites on the chromosome. Furthermore, the African gene employs a different method than the activator in the European gene.

Lactose persistence helps people to break down lactose in their bodies. Lactose persistence is caused by a mutation that occurs in African and European populations. The mutations share certain characteristics, including:

Lactose persistence is produced in both groups by single nucleotide polymorphism (SNP).

In both groups, the polymorphism does not appear in the LCT gene responsible for lactase production; rather, it occurs in the intron region of some other nearby gene that initiates the transcription of the LCT gene.

The following mutations differ in both populations:

Lactose persistence in the European population can be attributed to a single SNP in the intron region.

In the African population, numerous alleles are involved for lactose persistence in the intron region.

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combination (synthetic estrogen and progestin) hormonal birth control generally works by...?

Answers

Estrogen and progestin combinations function by inhibiting ovulation (the release of eggs from the ovaries). They also alter the uterine (womb) lining to prevent conception and alter the mucus at the cervix (uterine entrance) to inhibit sperm (male reproductive cells) from penetrating.

Hormonal birth control treatments that combine estrogen and progestin distribute estrogen and progestin throughout the body. These hormones primarily prevent conception by preventing pregnancy (the release of an egg from one of the ovaries). They also produce changes in the body that aid in the prevention of pregnancy.

The role of estrogen in combination with hormonal birth control is to regulate bleeding. Menstrual bleeding may change if only progestin is used. To make your "withdrawal bleed" look more like a "normal period," synthetic estrogens are added to these types of contraceptives.

COCs, often known as birth control tablets, provide dependable contraception as well as a number of noncontraceptive advantages.

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which of the following best describes a difference between generalist species and specialist species? responses generalists use a large range of resources, while specialists have a limited range of resources they use to survive.

Answers

The following best describes difference between generalist species and specialist species : generalists use large range of resources, while specialists have limited range of resources they use to survive.

What is meant by generalist and specialist species?

Generalist species may live in many different environments and eat a number of different things. Specialized species consume a more constrained diet and have considerably narrower ecological niches.

Specialist species are animals that require a certain set of resources. These animals either have a somewhat limited diet or need a specific habitat in order to survive. One example of an expert is the tiger salamander.

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suppose extracellular [k ] increases by 2.5 mm. if the original extracellular [k ] was 5 mm, how large (and in what direction) is the change in ek ?

Answers

The change in ek is +1.5 mV and it is in positive direction.

The Nernst equation is used to calculate the equilibrium potential (eK) of a given ion:

eK = (RT/zF)ln([K+]o/[K+]i)

Where R is the gas constant, T is the absolute temperature, z is the charge of the ion, F is Faraday's constant, [K+]o is the extracellular concentration of K+, and [K+]i is the intracellular concentration of K+.

In this case, we are given that the extracellular concentration of K+ increases by 2.5 mm. If the original extracellular concentration of K+ was 5 mm, the new extracellular concentration of K+ is 7.5 mm. We can plug these values into the Nernst equation to calculate the change in eK:

eK = (RT/zF)ln([K+]o/[K+]i)

eK = (RT/zF)ln(7.5/5)

eK = (RT/zF)ln(1.5)

eK = (RT/zF) × 0.405

eK = (RT/zF) × 0.405

eK = (8.314 × 300/1 × 9.648 × 104) × 0.405

eK = 1.5 mV

Therefore, the change in eK is +1.5 mV.

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Which of the following is a renewable energy source?
A.coal
B.natural gas
C.gasoline
D.solar power

Answers

Answer: Solar Energy

Explanation:

Other forms of energy like coal, natural gas and gasoline cannot be renewed by physical or mechanical methods. Also, it takes millions of years to form such non-renewable resources.

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