Answer:
In strict mode, the narrow-sense heritability is the proportion of the additive genetic variance that contributes to the total of the phenotypic variance. This value can be associated with the inheritance of the a-thalassemia .
Explanation:
A-thalassemia is a genetic disorder caused by mutations affecting four different genes that encode alpha-globin, thus affecting the hemoglobin production process and, consequently, oxygen transport. The mode of inheritance of the a-thalassemia may be associated with narrow sense heritability since the phenotype is manifested by gradation, i.e., each allele might contribute in similar mode to this genetic condition.
Based on the objective you should feel comfortable calculating expected genotype frequencies when given observed allele frequencies. You should also be able to compare expected genotype frequencies from HWE to observed frequencies and determine if evolution is occurring.Here's a sample problem. You can do this without a calculator!Let's take the noob gene, a gene with two known alleles that determines feather color in the eastern lark. The two alleles, let's call them delta and gamma, can be sequenced. In fact, looking at a population in southern GA, you discover that 60% of the alleles in the population are the delta allele.1. What is the percentage of the gamma allele in the population?2. Even more interesting, based on HWE what is the expected frequency of the population that is homozygous gamma? 3. Now, after surveying an isolated population of eastern larks, you find that 20% of the population is homozygous gama (gamma gamma), 65% heterozygous (gamma delta) and 10% homozygous delta (delta delta) and 5% of the population is a previously undescribed genotype - zeta zeta (homozygous zeta). What can you conclude about evolution in the population and why? If evolution is occurring, which mechanism(s) would be responsible?
Answer and Explanation:
60% of the alleles in the population are the delta allele
1. What is the percentage of the gamma allele in the population?
According to Hardy-Weinberg, the allelic frequencies in a locus are represented as p and q, referring to the allelic dominant or recessive forms. The genotypic frequencies after one generation are p² (Homozygous dominant), 2pq (Heterozygous), q² (Homozygous recessive). Populations in H-W equilibrium will get the same allelic frequencies generation after generation. The sum of these allelic frequencies equals 1, this is p + q = 1.
In the same way, the sum of genotypic frequencies equals 1, this is
p² + 2pq + q² = 1
Being
p the dominant allelic frequency, q the recessive allelic frequency, p²the homozygous dominant genotypic frequency q² the homozygous recessive genotypic frequency 2pq the heterozygous genotypic frequencyAccording to the sum of the allelic frequencies
p + q = 1
1 equals 100%
100% ------ 1
60%------0.6
40%------0.4
The percentage of gamma allele in the population is 40%.
2. what is the expected frequency of the population that is homozygous gamma?
The genotypic frequencies, F, after one generation are p² (Homozygous dominant), 2pq (Heterozygous), q² (Homozygous recessive).
F(gamma)= p²= 0.4²=0.16 =16%
F(delta)= q²= 0.6²=0.36 = 36%
F (gamma-delta) = 2xp2q= 2 x 0.4 x 0.6 = 0.48 = 48%
3. 20% of the population is homozygous gama (gamma gamma), 65% heterozygous (gamma delta) and 10% homozygous delta (delta delta) and 5% of the population is a previously undescribed genotype - zeta zeta (homozygous zeta). What can you conclude about evolution in the population and why? If evolution is occurring, which mechanism(s) would be responsible?
20% gamma-gamma
10% delta-delta
65% gamma-delta
5% zeta-zeta
This population has different genotypic frequencies with respect to the population in southern GA:
The gamma genotypic frequency is a little bit higher in the isolated population (16% vs 20%)
The delta genotypic frequency is considerably lower in the isolated population (36% vs 10%)
The heterozygotic genotypic frequency is also higher in the isolated population (18% vs 64%)
It appeared a new phenotype in the isolated population, probably due to a mutation in one of the genes. We can assume that this is a new mutation because its frequency is still low (5%).
Maybe, the mutation occurred in an individual of the isolated population, and with time, this mutation increased its frequency, up to a point of being phenotypically visible. This could be a case of a bottleneck example.
The founder effect is a special case of genetic drift, where a few organisms of one population settles down in a new area carrying the genes of the original population.
Genetic drift is the random change in the allelic frequency in a population, from one generation to the other. The magnitude of this change is inversely related to the size of the original population. These changes produced by genetic drift accumulate in time, and eventually, some alleles get lost, while some other might get set.
Founder effect refers to the consequences that occur from the origin of a new population that derives from a small number of founder individuals that come from an ancestral bigger population. This small group shows poor genetic variation and a high possibility of expressing a peculiar allelic composition. Some mutation might occur and it can accumulate in time.
Scenario: You are a research scientist and are working with eukaryotic cells obtained from a newly discovered organism, Oviductus oblatus. You want to determine if this organism’s cells are similar in structure an organization to other eukaryotic cells. You collect the cells and examine them using a light microscope and a cell stain in an attempt to see the internal cellular structures. Lo and behold you are happy because you are able to see particular structures using this method. You find the following features: Organism’s cell are able to move Internal structures appears to be made of protein fibers Internal structures protein fibers measured 7.0 nanometers diameter Internal structures protein fiber appears to be two strands intertwined with each others Based on this information you come to the conclusion that you have been able to identify in these cells the structure of ____. You conclude the cells of Oviductus oblatus are indeed (name the main type of cell it appears to be) ______ cells. Discussion: Post your answers for the above scenario and include evidence that supports your choice
Answer:
Based on this information you come to the conclusion that you have been able to identify in these cells the structure of microfilaments or actin filaments. Oviductus oblatus are indeed eukaryotic cellsExplanation:
In eukaryotic cells, the cytoskeleton is composed of three well defined filamentous structures: microtubules, microfilaments, and intermediate filaments. Each of these filamentous structures is a polymer of proteinic subunits united by weak, not covalent connections.
The microfilaments are the thinnest of the three structures. They have a diameter of 7 nanometers and are composed of many proteinic monomers united. This monomeric protein is called actin. Many monomers get combined to form a structure that assembles a double helix.
Due to the fact that these microfilaments are made of actin monomers, they are also known as actin filaments.
Actin filaments have directionality which means that their extremes have different structures.
In eukaryotic cells, genes that codify for actin microfilaments are highly conserved in all organisms, which is why they are often used as molecular markers for different studies.
The body can get water from milk, juices, fruits, and vegetables.
Please select the best answer from the choices provided.
ОТ
Ο Ε
Answer:
your answer is true
Explanation:
why do you think we tell you kids to eat your Vegetables
The body getting water from milk, juices, fruits, and vegetables is True.
What is Water?This is defined as a universal solvent and comprises of two elements known as Hydrogen and oxygen.
Water is present in all food forms and is usually extracted during digestion in the large intestine and transported to other parts of the body.
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Since the human genome was first published in 2001, scientists have quickly improved genetic sequencing, generating genetic and genomic data for hundreds of organisms. As data collection improves, the ability to collect and process whole genome sequences is becoming cheaper and easier. The prospect of sequencing entire genomes of many different species brings up many ethical questions related to both social issues and political policies. Select the statements related to the use of genetic information that are not potential social or ethical issues in the United States??1. Genetically modified organisms (GMO) are used to increase vitamin A content in maize.2. Databases are created using genetic sequence data collected at crime scenes.3. Participants willingly donate their DNA for specific research purposes.4. Approved genetic screening methods are used to detect rare disease alleles in afflicted individuals.5. Genomic sequence data is used to identify genetic variants that may impact an individual\'s response to different medicines.
Answer:
2. Databases are created using genetic sequence data collected at crime scenes.
3. Participants willingly donate their DNA for specific research purposes
Explanation:
Genetic sequence is also used for solving the criminal cases by using any biological material that is collected during investigation at the crime scene. The crime department has forensic lab where these biological materials are tested. Sometime participants also donate their DNA for specific research and experiments in order to increase the knowledge about genetics so both have no social or ethical issues in the United States.
Here is a hypothetical situation: An area of the country has received a record amount of rainfall this year coupled with a mild winter and a warm spring and summer. As a result the mosquito population is exceptionally high. Mosquitoes are not pathogens to humans. However, mosquitoes can greatly affect the rate of infection for several types of human pathogens. Pick and describe two pathogens that you should expect to see at higher frequency because of this booming mosquito population.
Answer:
Zika virus and West Nile virus are the two pathogens which causes more diseases due to increase the population of mosquito.
Explanation:
Zika virus belongs to the family of Flaviviridae. Aedes mosquitoes which are active at day time are the carrier of this virus. West Nile virus is also spread through the bite of mosquito. The infected mosquito is the main cause of spreading of this disease. This virus is spreading too fast in the United States of America in the summer season where the population of mosquitoes increases.
in what way do these trees suggest that some of these species of blood flukes have switched hots?
Answer:
Prevention. The basic means of preventing Schistosoma infection is avoiding contact with fresh water infested with Schistosome parasites. Swimming, wading, or any other aquatic activities in these bodies of water exposes the skin to possible penetration by the cercariae.Explanation:
true or false if you click on the name of a structure you identified incorrectly or correctly you will be taken to the module in APR where you can review this structure
Answer:
True.
Explanation:
When you are given a given structure and you ick on it to know if its correct or wrong you will be taken to a module on APR where u can review this structure and see clearly if it's right or wrong. That is how the website as be programmed. Once you click on it, u are automatically directed to APR to review and clarify your answers.
You come upon an outcropping of sandstone while working in the field in Southern Utah. You notice cross-beds. Which of the following would you be able to determine about this deposit, just from looking at it? Group of answer choices A.the complete mineral composition of the sandstone B.the direction of the wind at the time it was deposited C.the tectonic history of the deposit D.the origin of the sand in the deposit
Answer:
B. the direction of the wind at the time it was deposited
Explanation:
Cross beds refers to those rock beds which are formed due to the deposition of bed forms such as dunes. These cross beds are the group of inclined layer which is formed due to the flowing of water or wind. These rock beds represents the movement or direction of wind and water at the time of deposition and as a result cross beds are formed. So we can say that cross beds were formed due to the direction of wind.
Complete each sentence by dragging the proper word or phrase into the appropriate position. Then place each sentence in order from the largest to the smallest cavity size being described
Drag the text blocks below into their correct order.
1. The________ are found deep in the diaphyses of long medullary cavities bones and are filled with yellow marrow in the adult.
2. Osteocytes maintain contact with neighboring osteocytes in adjacent lamellae via dendrite-like extensions through __________
3. Filled with matrix, , which are osteoblasts encased in perforating canals are found between layers of concentric lamellae nutrient foramen
4. Located in the middle of osteons, the contain the osteocytes blood vessels, and nervous supply of the compact bones. osteons
lacunae
central canals
canaliculi
Answer:
The medullary cavities are found deep in the diaphyses of long medullary cavities bones and are filled with yellow marrow in the adult.
Osteocytes maintain contact with neighboring osteocytes in adjacent lamellae via dendrite-like extensions through canaliculi.
Filled with osteocytes, which are osteoblasts encased in matrix, lacunae are found between layers of concentric lamellae.
Located in the middle of osteons, the central canal contain the blood vessels, and nervous supply of the compact bones
Explanation:
- The medullary cavities are central cavities in the bones. In adults, these cavities are filled with yellow marrow (adipose tissue).
- The canaliculi are small protoplasmic projections between the lacunae of ossified bones.
- The astrocytes are the most common type of bone cell.
- The lacunae are spaces where osteocytes are located in the mature bones
- The central canal provides nourishment to the osteocytes which collectively form the osteon.
Which of the following statements is FALSE?
a.The Moon usually sets about 12 hours after it rises.
b.The Moon rises in the west and sets in the east.
c.The Full Moon rises around sunset.
d.The Moon takes a little more than 29 days to orbit the Earth.
The answer is b
Answer:
the answer is b
Explanation:
the sun sets in the south
II. PARTS OF THE DIGESTIVE TRACT A. ORAL CAVITY After ingestion, the physical breakdown of solid foods (mechanical digestion) begins in the oral cavity (mouth). Describe how food is mechanically digested in the oral cavity. Include a discussion of how the teeth, tongue, and saliva work together to convert solid food into a moist, semi-solid mass of food called a bolus. Example: think of how a hard, dry saltine cracker is converted into a moist ball (bolus) in the mouth. B. PHARYNX and ESOPHAGUS 1. After food has been mechanically digested, mixed with saliva, and a bolus has formed, it is swallowed. Swallowing moves the bolus from the mouth into the esophagus. Discuss the events that occur during swallowing. 2. Which portions of the pharynx does food pass through when swallowing
Answer:
This begins with the gradual breaking down of the food in the mouth, by grinding the food substances with the teeth this is mastication, and the secretion of saliva, which moisten the food, soften it so that the salivary amylase enzyme, carried out the mechanical digestion.
Swallowing is a wavelike muscular contraction which involve the contraction of the longitudinal and circular muscles of the oesophagus, which pushes the bolus down to the stomach.
The food pass Oropharynx. While the epiglottis prevents the bolus from entering the trachea.
This is followed by the rolling up of the tongue, the pushing the broken down food now chyme towards the back of the mouth, pressing it against the soft palate. At this stage a bolus is formed.From here it is pushed into the throat,where it is pushed down the oesophagus by peristalsis.
Explanation:
Give an example of a biological mutagen
Answer:
An example of a biological mutagen is Bacteriophage MU.
Other examples of mutagen are radioactive substances, ultraviolet radiation and certain chemicals
Which of the following is not a cause of water pollution?
a jet fuel
b laundry soap
c ammonia
d none of the above
Answer:
D) none of the above
Explanation:
took the quiz on edgenudity
The right answer is (a) jet fuel.
What is water pollution?Water pollution refers to the presence or introduction of harmful substances or pollutants into bodies of water such as lakes, rivers, oceans, and groundwater.
These pollutants can come from a variety of sources, including natural and human-made sources. While there are many different types of pollutants that can cause water pollution, some of the most common sources of water pollution include:
Industrial waste: Industries and factories release a variety of pollutants into water bodies, such as chemicals, heavy metals, and organic compounds.
Agricultural runoff: Fertilizers and pesticides used in agriculture can leach into waterways and cause significant pollution. Agricultural runoff can also contain soil, animal waste, and other contaminants that can degrade water quality.
Sewage and wastewater: Sewage and wastewater from homes and businesses contain a wide range of pollutants, including human waste, chemicals, and pharmaceuticals. These pollutants can have serious impacts on aquatic life and human health.
A jet fuel is not typically a cause of water pollution. While spills of jet fuel or other petroleum products can occur and can cause significant harm to aquatic ecosystems, they are not one of the most common causes of water pollution.
The most common causes of water pollution include things like industrial waste, agricultural runoff, sewage and wastewater, and improper disposal of household chemicals and medications.
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Viruses reproduce by .
the virus attaches itself to the host cell and inserts it's genetic material into it. then the host cell spreads the viruses genetic material which will result in spreading the virus.
The trait for medium-sized leaves in iris is determined by the genetic condition PP'. Plants with large leaves are PP, whereas plants with small leaves are P'P'. The trait for red flowers is controlled by the genes RR, pink by RR', and white by R'R'. A cross is made between two plants each with medium-sized leaves and pink flowers. If they produce 640 seedlings, what would be the expected phenotypes, and in what numbers would they be expected
Answer:
See the answer below
Explanation:
Medium size leaves genotype = PP'
Large leaves genotype = PP
Small leaves genotype = P'P'
Red flower genotype = RR
Pink flower genotype = RR'
White flower genotype = R'R'
Two plants each with medium-sized leaves (PP') and pink flowers (RR') were crossed.
PP'RR' x PP'RR' (let PP' = Aa and RR' = Bb)
Progeny and expected phenotypes
1 PPRR - large leaves, red flower = 1/16 x 640 = 40 seedlings
2 PPRR' - large leaves, pink flower = 2/16 x 640 = 80 seedlings
2 PP'RR - medium leaves, red flower = 2/16 x 640 = 80 seedlings
4 PP'RR' - medium leaves, pink flower = 4/16 x 640 = 160 seedlings
1 PPR'R' - large leaves, white flower = 1/16 x 640 = 40 seedlings
2 PP'R'R' - medium leaves, white flower = 2/16 x 640 = 80 seedlings
1 P'P'RR - small leaves, red flower = 1/16 x 640 = 40 seedlings
2 P'P'RR' - small leaves, pink flower = 2/16 x 640 = 80 seedlings
1 P'P'R'R' = small leaves, white flower = 1/16 x 640 = 40 seedlings
time._______________
is the relative temperature of a region over
O C. rain pattern
O B. climate
O A. weather
O D. wind currents
Answer: choice B. Climate
Explanation:
Climate is the relative temperature of a region over time.
7. A similarity between New Zealand and Australia is that- *
the native populations in both countries are the Aboriginies.
the monarch for both countries is Queen Elizabeth II.
neither country depends on tourism to boost their economy.
the nickname "Kiwi" refers to both Australians and New Zealanders.
Answer:
the monarch for both countries is Queen Elizabeth II.
Explanation: They are Island nations of the south Pacific.
The two countries were colonized by Abel Tasman, but were colonized by the British.Therefore recognized the Queen as the monarch. They both have a stable society and progressive movements. Both countries have good standard of living, with high sense of innovation and share almost the same type of food, wines, culture and music. They also have one of the best Diary farms in the world. They love the same sports-Rugby.
Help me ! Please man I need help !!
Which is a lymphocyte?
O A. Amylase
Ο Ο
B. Macrophage
O C. Urethra
D. B cell
Answer:
Lymphocyte is a B cell ans - option D2. Mr. Simpson has blood type ABRh+ and his wife type ORh+. Mr. Doodle has type ARh- and his wife type ORh+. The Simpsons and Doodles each had a baby girl on the same day. When they took their children home, their doctors found that the Doodle girl had blood type ARh- and the Simpson girl had type ORh+. The Simpsons sued the hospital for giving them the wrong baby. Could they win the case? Show the most likely genotypes of the parents and of the 2 girls
Interplanetary material that enters the earths atmosphere and collides with the ground rather than burning up is called
Explanation:Meteorito o meteoro
All 113 baby mice in an experiment are Rr, the parents are probably (do a Punnett square to be sure)
a
RR x RR
b
RR x rr
c
Rr x Rr
Answer:
The correct answer is option B.
Explanation:
If am individual cross between two true breed mice the probability of offspring with heterozygous condition is 100 percent in such cross. True breed is an individual organism with homozygous for the particular character either dominant or recessive.
So, if mice with RR (purebred or homozygous dominant) bred with mice with rr (homozygous recessive), all the offspring will be heterozygous in genotype which is Rr.
Thus, the correct answer is : option B. (Punnet square is attached)
Answer:
B
Explanation:
1. The amount of adenine is equal to the amount of thymine, and the amount of guanine is equal to the amount of uracil in RNA.
True or False?
2. The correct steps of the scientific methods are: experiment, conclusion, exploration, application
True or False
Answer:
1,True
Explanation:
2, exploration -experiment-application -conclusion
1) False because thymine is absent in RNA.
2)False, correct order is exploration, experiment, application, and conclusion. Hence both statements are false.
What is RNA?RNA (ribonucleic acid)is a nucleic acid, present in almost all organisms. It is single-stranded. In some organisms, it acts as genetic material. It constitutes of a ribose sugar, phosphate group, and nitrogen base and that are adenine, uracil, guanine, and cytosine. Uracil is present in RNA in place of thymine.
There are three types of RNA that are mRNA, tRNA, and rRNA. It does not obey Chargaff's rule. It has an important role in protein synthesis by ribosomes through the translation process.
The correct order of scientific method is exploration, experiment, application, and conclusion. Hence both statements are false.
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In the lab on Trophic Interactions you were looking at behavior of mosquito larvae. The question being asked was: Does prey behavior change in the presence of a predator? What would be an appropriate null hypothesis for this experiment? Recall in Treatments 2 and 3, a predator was added to the mosquito tanks.
Answer:
Null hypothesis: the behavior of mosquito larvae doesn't change in the presence/absence of predators added to the tanks in Treatments 2 and 3.
Explanation:
In statistics, the null hypothesis states that there are no differences between the two variables tested in the experiment, while the alternative hypothesis uses the experimental data in order to reject the null hypothesis. In this case, the alternative hypothesis will use the data from treatments 2 and 3 to confirm that predators can modify the behavior of mosquito larvae.
You are running an experiment to study seed germination and accidentally left a dish of seeds covered with a little water in a totally dark room. When you return a few days later, you notice that the seeds have sprouted long roots and long shoots. What was the source of most of the matter that makes up these roots and shoots?A. oxygen in the airB. carbon dioxide in the airC. water in the dishD. glucose in the seed
Answer:
D. glucose in the seed
Explanation:
Plants generally make their food through the process of photosynthesis in the presence of sunlight by utilizing carbon-dioxide and water and produces oxygen and carbohydrate.
But due to the absence of sunlight, plant will be unable to use carbon dioxide and water for photosynthesis and oxygen is a by-product.
So the reason of seed germination is the presence of glucose in the seed. Seeds generally germinate through the process of inhibition, that require uptake of water. Glucose works as energy source in humans and plants as well. sugar or glucose allow the seed to utilize the water and help foster its growth.
Hence, the correct option is D. glucose in the seed.
13. Ranchers lobbied the government to eradicate wolves from Yellowstone National Park. Five years later the deer population had increased fivefold. The following winter 70% of the deer starved to death. Explain why this was bound to happen.
Answer: The overgrowth in deer population led to a food shortage.
Explanation:
Since the eradication of the wolves, the deer had no predator to keep their total population in check. Due to the vast amount of deer the food in the area was being eaten at a faster rate. Most of the deer died do to their environment not being capable of feeding that many deer.
(I hope this helps! I tried my best.)
In lab we looked at succession using marine organisms. The process of succession also happens because of wildfires, and those communities do recover and follow the general model of succession. Below is a photo I took one year after a fire at Lake Tahoe. You can see see the burned trees in this photo. What proportion of r-selected species relative to K- selected species should we expect to see at this early stage of recovery?
The given question is not complete, the complete question is:
In lab we looked at succession using marine organisms. The process of succession also happens because of wildfires, and those communities do recover and follow the general model of succession. Below is a photo I took one year after a fire at Lake Tahoe. You can see see the burned trees in this photo. What proportion of r-selected species relative to K-selected species should we expect to see at this early stage of recovery? a. # of r-selected species > # of K-selected species b. # of r-selected species = # of K-selected species c. # of r-selected species < # of K-selected species. The diagram is given in the attachment below.
Answer:
The correct answer is option A, that is, the population of r-selected species will be more in comparison to the k-selected species.
Explanation:
Based on the evolutionary strategies, the preferred selection of the species that generate small body sizes, short life span offspring, and exhibit early semelparity and thrive in unstable surroundings is r-selection. These species possess the tendency to overcome the huge loss of offspring and are considered opportunists.
On the other hand, the preferred selection of the species that exhibit delayed reproduction, generate few large body size, possess long life span offspring and iteroparity, and thrive in stable surroundings is k-selection. Thus, it is clear that the r-selected species will be more in number than the species that are k-selected as r-selection plays an essential role in the initial phases of succession.
List the functions of kinases and cyclins, and describe how they interact to cause cells to move through the cell cycle. Drag the terms on the left to the appropriate blanks on the right to complete the sentences.
The provided question is incomplete as drag and drop box or required image is not available, however, the correct image of drag drop box is attached with the answer:
Answer:
Kinases are catalysts or enzyme that regulates the organic action of proteins by adding phosphate groups and cause the conformation change to a dormant to a functioning type of the protein.
Cyclins bind to CDK or cyclin dependent kinases to regulate by switching on and off the progression of the cell through cell cycle. At the G2 mitosis border , a CDK 1 combines with another cyclin ( B) , phosphorylation (adding of phosphate group) occurs bringing about a series of changes in the nuclear membrane via cytoskeleton, caldesmon and histone H1. CDK 4 binds to cyclin D , moving cells from G1 to S.
During digestion fat is broken down into
Answer:
Lipase
Explanation:
Lipase enzymes break down fat into fatty acids and glycerol. Digestion of fat in the small intestine is helped by bile, made in the liver.
In the parental generation, inbred black and an albino mice parents mate. The resulting F1 mice are black and heterozygous at 2 different loci. The F2 population consists of black, white, and albino mice. Albino is epistatic to pigmented. What is the phenotypic segregation ratio of this mouse species in the F2 generation? Enter your answer in the format: "4:3:2". Note lack of color pigmentation is recessive.
Answer:
12:4:3
Explanation:
Let the lack of color pigmentation which is recessive be aa where AA and Aa allow for color (BB, Bb - black, baby - white).
The F1 parents are BbAa x BbAa
This cross will produce 12/16 blacks, 4/16 albinos and 3/16 white.
Name a serious disease caused by protozoa
Answer:
African sleeping sickness is caused by protozoa in the Trypanosoma genus. These organisms are carried by the tsetse fly and transmitted to humans via tsetse fly bites. According to the World Health Organization, Trypanosoma brucei gambiense causes more than 90 percent of African sleeping sickness cases.
