The sample concentration was measured at 50mg/ml. The loading concentration needs to be 10mg/ml. The final volume needs to be 25ul. What is the volume of sample needed and the amount of buffer needed to reach 25ul

Answers

Answer 1

Answer:

a)  [tex]V_1=5ul[/tex]

b)  [tex]v=20ul[/tex]

Explanation:

From the question we are told that:

initial Concentration [tex]C_1=50mg/ml[/tex]

Final Concentration [tex]C_2=10mg/ml[/tex]

Final volume needs [tex]V_2 =25ul[/tex]

Generally the equation for Volume is mathematically given by

[tex]C_1V_1=C_2V_2[/tex]

[tex]V_1=\frac{C_1V_1}{C_2}[/tex]

[tex]V_1=\frac{10*25}{50}[/tex]

[tex]V_1=5ul[/tex]

Therefore

The volume of buffer needed is

[tex]v=V_2-V_1\\\\v=25-5[/tex]

[tex]v=20ul[/tex]


Related Questions

In order to complete the reaction of hexyl magnesium bromide with acetone, what next step needs to be done.
a. Fractional Distillation.
b. Vacuum filtration.
c. Aqueous workup.
d. Crystallization.

Answers

Answer:

Aqueous workup.

Explanation:

The reaction of hexyl magnesium bromide with acetone yields a tertiary alcohol. There is an organic phase and an aqueous phase.

Aqueous workup is the process of recovering the pure tertiary alcohol from the organic phase of the system.

Hence, in order to complete the reaction of hexyl magnesium bromide with acetone, aqueous workup is required.

What test was used to identify hydrogen gas? Write a balanced equation to represent the test?

Answers

Burning splint test
2H2 (g) + O2 (g) -> 2H20 (g) + heat
It’s a combustion reaction

Convert 1.16 mol of calcium cyanide, Ca(CN)2, to mass.

Answers

here it the answers in pencil

How many oxygen atoms are there in 0.25 mole of CO2?

Answers

Answer:

It is known that 1 mol of a molecule contains 6.023×1023 6.023 × 10 23 number of molecules. So, 0.25 moles of CO2 C O 2.

The correct option for the given question about Mole Concept is Na / 2 atom of oxygen.

What is mole?In chemistry, a mole is a unit. A mole of a substance is the mass of the substance containing precisely 12 gram of the same number of atoms as 12C.The mole is the SI unit used to measure a substance's quantity. It has the symbol mol.

How to solve this question?

In 1 mole number of molecules of CO₂ = Na (Avagadro Number)

In 0.25 mole number of molecules of CO₂ = 0.25 × Na molecules

In 1 molecule number of oxygen atom = 2 atom

In 0.25 × Na molecules number of oxygen atom = 2 × 0.25 × Na atom

In 0.25 × Na molecules number of oxygen atom = 0.5 × Na atom

So in 0.25 mole of CO₂ number of oxygen atom = Na / 2 atom

Thus we can conclude that in 0.25 mole of CO₂ number of oxygen atom will be Na / 2, where Na is Avagadro Number.

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what happens if we mix vitamin c and d together

Answers

we can get rid of scurvy as well as rickets ......

4) Calculate the percentage by
mass of oxygen in Pb(NO3)2
A) 193
B20
145

Answers

Answer:

  im getting 331.23

Explanation:

Element Number Mass Percet Composition

O            6         16.00  28.9828819853274

N             2         14.01 8.45937867946744

Pb             1        207.21 62.5577393352051

Which phenomena does wind cause?

A)tides and waves

B)deep currents and tides

C)waves and surface currents

D)surface currents and deep currents

HELP PLEASE ILL GIVE OUT BRAINLIEST

Answers

A) tides and waves.

The phenomena which  wind causes  are waves and surface currents.

What are surface  currents?

Surface currents are caused by  winds  which blow over the ocean water and result in creation of waves. Surface currents  are horizontal streams of water which are capable of  flowing thousands of kilometers and can reach great depths.

Surface currents play a major role in determining the climate all over the world. They are influenced by three major factors 1) overall global wind patterns 2)rotation of earth 3)shape of ocean basins . When a surface  current collides with the land it  changes the direction of currents.

Because of surface currents the global climate has warmer water  from equator to the cooler parts of the ocean  as they transfer heat energy.

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LAST ATTEMPT! Covalent compound naming ! !!

Answers

Answer:

Name the non-metal furthest to the left on the periodic table by its elemental name.

Name the other non-metal by its elemental name and an -ide ending.

Use the prefixes mono-, di-, tri-.... to indicate the number of that element in the molecule.

im prolly wrong

what is wrong with the statement? "because there are no common factors in the molecular formula, k2co3 is also the empirical formula of potassium carbonate"

Answers

The empirical formula is the same as the molecular formula because the ratio of atoms in the compound correspond to the number of atoms of each element in the compound.

The empirical formula of a compound shows the ratio of atoms of each element in the compound while the molecular formula shows the number of atoms of each element in the compound.

For the compound, K2CO3, the empirical formula is the same as the molecular formula because the ratio of atoms in the compound correspond to the number of atoms of each element in the compound. It has nothing to do with common factors.

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Which condition is usually associated with low air pressure systems?

Answers

Answer:

clouds and precipitation that minimize temperature changes throughout the day

Explanation:

increasing temperature increases:
1:Enthalpy
2:Free Energy
3:Entropy
4:Specific heat​

Answers

Answer:

2 :free energy

Explanation:

I hope it is fine answer for you ☺️☺️☺️

What is the empirical formula for a compound that is comprised of 54.0% sodium, 8.50% boron, and 37.5% oxygen by mass

Answers

Answer:  

Sodium metaborate: sodium+boron+oxygen= NaBO 2.

The formula can be written also as Na 2O·B

Explanation:

Pure silver has a brilliant white metallic luster. How many moles of silver, Ag, are in 7.54 × 1023 atoms of silver?

Answers

The number of moles of silver, Ag, that are in 7.54 × 10²³ atoms of silver is 1.25 moles

From the question,

We are to determine the number of moles of silver, Ag, that are present in 7.54 × 10²³ atoms of silver

From the formula

[tex]Number\ of\ moles =\frac{Number\ of\ atoms}{Avogadro's\ constant}[/tex]

Avogadro's  constant = 6.022 × 10²³  atoms mol⁻¹

and

From the given information

Number of silver atoms = 7.54 × 10²³ atoms

Putting the parameters into the formula, we get

Number of moles of silver present = [tex]\frac{7.54\times 10^{23} }{6.022\times 10^{23}}[/tex]

Number of moles of silver present = 1.2520757 moles

Number of moles of silver present ≅ 1.25 moles

Hence, the number of moles of silver, Ag, that are in 7.54 × 10²³ atoms of silver is 1.25 moles

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Which combination of reagents used in the indicated order with benzene will give m-nitropropylbenzene? a. 1) CH3CH2CH2Cl/AlCl3, 2) HNO3/H2SO4 b. 1) HNO3/H2SO4, 2) CH3CH2CH2Cl/AlCl3 c. 1) CH3CH2CH2Cl/AlCl3, 2) HNO3/H2SO4, 3) Zn/HCl d. 1) HNO3/H2SO4, 2) CH3CH2CH2Cl/AlCl3, 3) Zn/HCl

Answers

Answer:

1) HNO3/H2SO4, 2) CH3CH2CH2Cl/AlCl3

Explanation:

Benzene is a stable aromatic compound hence it undergoes substitution rather than addition reaction.

When benzene undergoes substitution reaction, the substituent introduced into the ring determines the position of the incoming electrophile.

If I want to synthesize m-nitropropylbenzene, I will first carry out the nitration of benzene using HNO3/H2SO4 since the -nitro group is a meta director. This is now followed by Friedel Craft's alkykation using CH3CH2CH2Cl/AlCl3.

Please write out the balanced chemical equations for the following
reactions.
**Ensure you write the states of the reactants and products.**
• zirconium chloride reacts with scandium dichromate

Answers

Answer: Super idol

Explanation:

chinese malapoks

PLEASE HELP ME!! sue tomorrow:(

Answers

Answer:

If you are looking for the answer, for the question.

Earthquakes trigger tsunamis when the seismic activity causes the land along fault lines to move up or down

What is the job of cellular respiration?

Answers

Answer:

to break down sugar in the presence of oxygen to release energy in the form of ATP

Explanation: i just finished this chapter of the class

Choose the compound below that contains at least one polar covalent bond, but is nonpolar.
a. SiF4
b. SeF4
c. HCl
d. ICl3
e. Both SiF4 and ICl3 are nonpolar and contain a polar covalent bond.

Answers

Answer:

HCl contain a polar covalent bond because H is more electropositive than Cl and Cl is more electronegative than H, resulting in a dipole moment

in a test trial, using 6% extra strength bleach at room temperature, 100.0 mL of oxygen gas was produced in 2.03 seconds. Calculate the average reaction rate for the oxygen gas.

Answers

The average reaction rate for oxygen gas is 49.26 mL/s

The average reaction rate of a substance refers to the speed at which a reaction occurs and therefore products are formed. In the reaction presented, oxygen gas is the product.

Moreover, the average reaction rate can be expressed in mL per second or mL/s, and the formula to calculate it is:

Product in mL/seconds

Let's calculate the reaction rate:

100 mL/ 2.03 s = 49.26 mL/s

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30 points!!!!

2K + 2H2O → 2KOH + H2

This reaction shows which detail about potassium and hydrogen?
Hydrogen is more reactive than potassium.
Potassium and hydrogen form ionic bonds.
Potassium is more reactive than hydrogen.
Potassium and hydrogen form covalent bonds

Answers

Answer:

2K + 2H2O → 2KOH + H2

So Hydrogen is more reactive than pottasium .

According to law of conservation of matter, 2 moles of potassium react with water to produce 1 mole of hydrogen gas.

So, 2 moles of potassium produce 1 mole of hydrogen gas.

Answer:

A

Explanation:

Hydrogen is more reactive than potassium.

An hibernating bear accumulates 25 kg of a triglyceride (846 g/mol) comprising the following fatty acids: C16, C17 and C18∆9. How many grams of β-hydroxybutyrate (104 g/mol) can the bear produce from this triglyceride during its hibernation?

Answers

The quantity of β-hydroxybutyrate the bear can produce

during hibernation is 3,073.30grams.

Hibernation is defined as the period of less activity by animals to conserve energy to survive coarse weather conditions.

A typical example of an animal that hibernates is the bear.

From the question, a bear is able to accumulate 25kg (25000grams) of triglyceride.

To calculate quantity of β-hydroxybutyrate that would be produced from 25kg triglyceride;

846g of triglyceride = 104grams of β-hydroxybutyrate

25000g of triglyceride = x grams

(Cross multiple to solve for x grams)

X = 104 × 25000/846

[tex]x = \frac{104 \times 25000}{846} [/tex]

[tex]x = \frac{2600000}{846} [/tex]

[tex]x = 3073.30grams[/tex]

Therefore, the quantity of β-hydroxybutyrate the bear can produce during hibernation is 3,073.30grams.

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When comparing Be2 and H2:

I. Be2 is more stable because it contains both bonding and antibonding valence electrons.
II. H2 has a higher bond order than Be2.
III. H2 is more stable because it only contains 1s electrons.
IV. H2 is more stable because it is diamagnetic, whereas Be2 is paramagnetic

a. II,III,IV
b.II,III
c.III only
d.I,II
e.III,IV.

Answers

Answer:

The answer is "Option b".

Explanation:

H2 does have bond energy of 1, while Be2 has a covalent bond of zero. Be2 has eight electrons, each of which dwells in a distinct orbital. As just a result, four of them are linked molecular orbitals and two are antibonding molecular orbitals, respectively. As just a result, this molecule is unstable. This chemical orbital, with a bond order of 1, has just two electrons. As a result, it is a very solid substance. H2's bond length is higher than Be2's. Since it only has one electron, H2 is more stable than that of other compounds.

How many moles is 7.78 x 1024
formula units of MgCl2?

Answers

How many moles is 7.78 x 1024

formula units of MgCl2?

answer: 7966.72

Mole measure the number of elementary entities of a given substance that are present in a given sample. Therefore, there are 0.49moles of  MgCl[tex]_2[/tex] in  7.78 x 10²⁴atoms of MgCl[tex]_2[/tex] .

What is mole?

The SI unit of amount of substance in chemistry is mole. The mole is used to measure the quantity of amount of substance. It measure the number of elementary entities of a given substance that are present in a given sample. There are so many formula for calculating mole.

we know one mole of any element contains 6.022×10²³ atoms which is also called Avogadro number

mole =given number of atoms ÷ 6.022×10²³(Avogadro number)

given number of atoms of MgCl[tex]_2[/tex]= 7.78 x 10²⁴

Substituting the values

mole= 7.78 x 10²⁴÷ 6.022×10²³

mole = 0.49moles of  MgCl[tex]_2[/tex]

So, there are 0.49moles of MgCl[tex]_2[/tex] in  7.78 x 10²⁴atoms of MgCl[tex]_2[/tex] .

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What is always true about lenses?

Answers

Answer: They all bend light.

i don't know much about lenses so i hope this helps! :)

What equipment is generally used to make lyophilized medications suitable for administering to the patient? a) Test tubes or sterile ampules O b) Petri dishes and sterile droppers c) Sterile syringes or graduated cylinders d) Measuring cups and clean, warm water

26. Which group in the periodic table contains
an element that can form a blue sulfate
a
compound?
(1) 1
(2)
(3) 11
(4) 17

Answers

Answer:

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Answer:

Option (3) 11

Explanation:

Elements in Group 11 of the periodic table are called Noble Metals.Noble metals have high resistance to oxidation and low chemical reactivity.Noble metal includes Gold (Au), Silver (Ag), Copper (Cu).Copper forms a blue sulfate when reacts with Sulphuric Acid.Formula of Copper Sulfate : CuSO4.Since, Copper belongs to the Group 11 elements of the periodic table hence, option (3) is the right answer.

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Define atomic numbers and mass numbers

Answers

Atomic numbers is the number of a chemical element in the periodic table and the mass number is the total number of protons and neutrons in an atom

Answer:

1). Atoms of each element contain a characteristic number of protons. In fact, the number of protons determines what atom we are looking at (e.g., all atoms with six protons are carbon atoms); the number of protons in an atom is called the atomic number.

2) In contrast, the number of neutrons for a given element can vary. Forms of the same atom that differ only in their number of neutrons are called isotopes.

3) Together, the number of protons and the number of neutrons determine an element’s mass number: mass number = protons + neutrons. If you want to calculate how many neutrons an atom has, you can simply subtract the number of protons, or atomic number, from the mass number.

convert 8.4 • 10^16 molecules of CO2 to moles

Answers

Answer:

0.00000000000000084

Explanation:

How can a redox reaction be identified?
O A. Atoms have rearranged.
O B. Protons have been transferred.
.
O
C. lons are present in solution.
D. Oxidation states have changed.

Answers

Answer:

'Oxidation states have changed.'

Explanation:

Redox Reactions can be identifiable by a change in oxidation number/states of two of the atoms in the reaction. Any reaction in which no oxidation numbers/state change is not a redox reaction.

Element X has an electron configuration of 2-8-2. This element will combine with the oxide ion to form a compound with the formula

Answers

2+8+2 = 12 electrons
Magnesium has 12 electrons
Magnesium and oxide combine to for Magnesium Oxide
Mg^2+ + O^2- = MgO

An analytical chemist is titrating of a solution of benzoic acid with a solution of . The of benzoic acid is . Calculate the pH of the acid solution after the chemist has added of the solution to it. Note for advanced students: you may assume the final volume equals the initial volume of the solution plus the volume of solution added.

Answers

The question is incomplete. The complete question is :

An analytical chemist is titrating 148.9 mL of a 1.100 M solution of benzoic acid [tex]$HC_6H_5CO_2$[/tex]  with a 0.3600 M solution of KOH. The [tex]pK_a[/tex]  of benzoic acid is 4.20. Calculate the pH of the acid solution after the chemist has added 232.0 mL of the KOH solution to it. Note for advanced students: you may assume the final volume equals the initial volume of the solution plus the volume of KOH solution added.

Solution :

Number of moles of [tex]$C_6H_5OCOOH$[/tex] [tex]$=148.9 \ mL \times \frac{L}{1000\ mL} \times \frac{1.100 \ mol}{L}$[/tex]

                                                         = 0.16379 mol

Number of moles of NaOH added [tex]$=232.0 \ mL \times \frac{L}{1000\ mL} \times \frac{0.3600 \ mol}{L}$[/tex]

                                                         = 0.08352 mol

ICE table :

                     [tex]C_6H_5OCOOH \ \ \ + \ \ \ OH^- \ \ \ \rightarrow \ \ C_6H_5OCOO^- \ \ \ \ + \ \ H_2O[/tex]

I (mol)              0.16379                 0.08352              0

C (mol)           -0.08352              -0.08352            +0.08352

E (mol)            0.08027                    0                    0.08352

Total volume = (148.9 + 232) mL

                      = 380.9 mL

                     = 0.3809 L

Concentration of [tex]$C_6H_5OCOOH, [C_6H_5OCOOH]$[/tex] [tex]$=\frac{0.08027 \ mol}{0.3809 \ L}$[/tex]

                                                                               = 0.211 M

Concentration of [tex]$C_6H_5OCOO^- , [C_6H_5OCOO^-] =\frac{0.08352 \ mol}{0.3809 \ L}[/tex]

                                                                              = 0.219 M

[tex]pK_a[/tex] of [tex]C_6H_5OCOOH = 4.20[/tex]

According to Henderson equation,

[tex]$pH = pK_a + \log \frac{[C_6H_5OCOO^-]}{[C_6H_5OCOOH]}[/tex]

     [tex]$=4.20 + \log \frac{0.219}{0.211}$[/tex]

     = 4.22

Therefore, the pH of the acid solution is 4.22

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