Some enzymes have one or more sulfhydryl (thiol) groups that are important to enzymatic activity but that can react upon standing in solution to form inactive disulfide bonds.
Thiol reagents, such as 1,4-dithiothreitol (DTT), are often added to the solutions of such proteins to protect them from this reaction and to reverse it when it occurs. (The reverse reaction works best at slightly alkaline pH.) Draw the product formed when DTT reacts with a protein disulfide bond to liberate the free thiol groups. Which of the following occurs in this reaction? A. The protein disulfide is oxidized.
B. The protein disulfide is reduced.
C. DTT is reduced.
D. DTT is oxidized.

Answers

Answer 1

Answer:

A. Protein disulfide is oxidized.

Explanation:

When thiol reagents are introduced with some protein solutions they react with molecules of disulfide and oxidize the protein. There occurs inter-conversion of thiol molecules into free disulfide molecules. The DTT reduces the disulfide molecules bonds of proteins and it starts to peptide.


Related Questions

What makes up nearly all of the atom's mass?
A. The sum of all protons and neutrons
B. The sum of all neutrons and electrons
C. The sum of all protons and electrons
D. The sum of all isotopes
SUBMIT

Answers

Answer:

a. the sum of all protons and neutrons

A the sum of all protons and neutron

What is a relief in relation to topography ?

Answers

Answer:

Relief is typically defined as the difference in height between the high point and the low point on a landscape, in feet or in meters.

Explanation:

Answer:

Relief is the difference in elevation between two points. It is easily calculated by subtracting the lowest elevation from the highest elevation in an area.

Explanation:

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what is The pH and pOH of a 1.00x10-3M solution of CH3COOH (Ka=1.75x10-5) ?

Answers

Answer:

pH = 3.9pOH = 10.1

Explanation:

Since [tex]CH _ 3COOH[/tex] is a weak acid to find the pH of [tex]CH _ 3COOH[/tex] we use the formula

[tex]pH = - \frac{1}{2} log(Ka) - \frac{1}{2} log(c) [/tex]

where

Ka is the acid dissociation constant

c is the concentration

From the question

Ka of [tex]CH _ 3COOH[/tex] = 1.75 × 10^-5

c = 1.00 × 10-³M

Substitute the values into the above formula and solve for the pH

That's

[tex]pH = \frac{1}{2} ( - log(1.75 \times {10 }^{ - 5} - log(1.00 \times {10}^{ - 3} ) ) \\ = \frac{1}{2} (4.757 + 3) \\ = \frac{1}{2} \times 7.757) \\ = 3.8785 \: \: \: \: \: \: \: \: [/tex]

We have the answer as

pH = 3.9

To find the pOH we use the formula

pH + pOH = 14

pOH = 14 - pH

pOH = 14 - 3.9

We have the answer as

pOH = 10.1

Hope this helps you

What is the equation that describes the relationship of force, mass, and acceleration?

Answers

Answer:

f=ma

Explanation:

Force is measured in newtons btw

Most people use the _____ for intercity travel

Answers

if your options are
train
subway system
domestic airports
private bus system

the correct answer is private bus system

Answer:

The person at the top said the right answer I checked

why are there so many ads

Answers

Ads are a source of revenue for many websites, it also brings popularity to the things the ad portrays which means better business all around.
Some websites have so many popup ads because it makes them more money and it's relatively easy to implement. But this isn't something every web site can or should do. It depends on the Ad network they are using or the type of Ads. There are Ad networks which offer pop ups for website publishers.

Calculate the net force that thymine exerts on adenine. To keep the calculations fairly simple, yet reasonable, consider only the forces due to the O−H−N O − H − N and the N−H−N N − H − N combinations, assuming that these two combinations are parallel to each other. Remember, however, that in the O−H−N O − H − N set, the O− O − exerts a force on both the H+ H + and the N− N − , and likewise along the N−H−N N − H − N set.

Answers

Answer:

hello your question is incomplete and lacks the required figure  below is the missing part

The two sides of the DNA double helix are connected by pairs of bases (adenine, thymine, cytosine, and guanine). Because of the geometric shape of these molecules, adenine bonds with thymine and cytosine bonds with guanine shows the thymine adenine bond. Each charge shown is ±e, and the H−N distance is 0.110 nm

answer : net force due to O-H-N combination = -5.03354 * 10^-9 N

              net force due to N-H-N combination = -3.822 * 10^-9 N

              net force that the thymine exerts on adenine =  -8.8558 * 10^-9 N

Explanation:

considering only the forces due to O-H-N and N-H-N to determine the net force been exerted on adenine by thymine

we have to make some assumptions

we have to assume force towards thymine to be negative

Net force that thymine exerts on Adenine

= net force due to 0-H-N  + net force due to N-H-N

= ( -5.03354 * 10^-9 ) +  ( - 3.822 * 10 ^-9 )

=  -8.8558 * 10 ^-9 N

ATTACHED BELOW IS THE REMAINING PART OF THE SOLUTION

Heptane and water do not mix, and heptane has a lower density (0.684 g/mL) than water (1.00 g/mL). A 100-mL graduated cylinder with an inside diameter of 3.16 cm contains 34.6 g of heptane and 34.0 g of water. What is the combined height of the two liquid layers in the cylinder? The volume of a cylinder is π r2h, where r is the radius and h is the height.

Answers

Answer:

[tex]h=100.8cm[/tex]

Explanation:

Hello,

In this case, considering the density and mass of both water and heptane we first compute the volume of each one:

[tex]V_{water}=\frac{m_{water}}{\rho _{water}}=\frac{34g}{1.00g/mL}=34mL\\ \\V_{heptane}=\frac{m_{heptane}}{\rho _{heptane}}=\frac{34.6g}{0.684g/mL}=50.6mL\\[/tex]

Now, the total volume is:

[tex]V=50.6mL+34mL=84.6mL[/tex]

Which is equal to:

[tex]V=84.6cm^3[/tex]

Then, by knowing that the volume of a cylinder is πr²h or π(D/2)²h, we solve for the height as follows:

[tex]h=\frac{V}{\pi (D/2)^2} \\\\h=\frac{84.6cm^3}{\pi (3.16cm/2)^2} \\\\h=100.8cm[/tex]

Best regards.

True or False:
Because the length, width and height of a cube are both
extensive properties, the volume of the cube is also an
extensive property.

Answers

Answer:

true

Explanation:

is Crystallizing sodium Acetate a physical or chemical change

Answers

Answer: physical please let me know if its wrong !

Which Of The Following Monosaccharides Is Not An Aldose? A) Crythrose B) Fructose C) Glucose D) Glyceraldehyde E) Iribose

Answers

A) Crythrose is not an Aldiss

Fructose monosaccharides is not an aldose sugar. Hence option B is correct.

What is Monosaccharide?

Monosaccharide is a simplest form of sugar that is not decompose in simple sugars by hydrolysis and it contains one or more hydroxyl groups.

Types of sugars Aldose and ketose (which are named on the basis of presence of functional groups aldehyde and ketone).

Aldose is a monosaccharide contains carbon chain with carbonyl group in endmost carbon atom.

Erythrose, glucose, glyceraldehyde and ribose contain aldehyde as a functional group so these are example of aldose where as fructose contains a ketone group so it is a ketose not an aldose.

Fructose monosaccharides is not an aldose sugar. Hence option B is correct.

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Draw the structure of aspirin acid and conjugate base.

Answers

Answer:

Aspirin = acetylsalicyclic acid

Its conjugate base = acetylsalicyclate

Note: See the attachment below for structures.

Explanation:

Aspirin, also known as acetylsalicyclic acid is derived from the acetylation of salicyclic acid at carbon-2. It is used as a medication in the treatment of pain, fever and inflammation.

It is grouped under the class of non-steroidal anti-inflammatory drugs (NSAIDs), which are a class of drugs that in addition to anti-inflammatory actions,  have analgesic, antipyretic, and platelet-inhibitory actions.

Its conjugate base, acetylsalicyclate is obtained from the deprotonation of the carboxylic acid group, -COOH.

if you add sugar to water and the sugar no longer dissolves and it settles at the bottom the solution has become

Answers

Answer:

It forms a solution.

Explanation:

A pure substance is when only one type of atom or molecule is present. In this case the substance contains two different molecules; sugar and water, assuming sugar is all one type (e.g. glucose) and there are no contaminants in the water or sugar before combining the two.

Dissolving the sugar in the water makes a homogeneous mixture and once dissolved a solution is made by the combination of a solute (sugar) and solvent (water).

Hope this helps!!!!

If you start a chemical reaction with 38 grams of reactants, how many grams of a product would
you end up with?
Your answer:

Answers

Answer:

38 g as well due to the law of conservation of mass.

Explanation:

Hello,

In this case, we need to review the law of conservation of mass which states that matter cannot be neither created nor destroyed, thus, for chemical reactions, the mass of reactants at the beginning of the chemical reaction must equal the mass of products at the end in closed systems, that is why, since 38 g is the mass of reactants, the mass of the overall products is also 38 g.

Best regards.

1/2-life of pyruvic acid in the presence of aminotransferase enzyme (which converts it to alanine) was found to be 221 s. how long will it take for the concentration of pyruvic acid to fall to 1/64 of its initial value in this first order reaction?

Answers

Answer:

After 1326s, the concentration of pyruvic acid fall to 1/64 of its initial concentration.

Explanation:

The first order kinetics reaction is:

ln [A] = ln [A]₀ - kt

Where [A] is concentration after t time, [A]₀ is intial concentration and k is reaction constant.

To convert half-life to k you must use:

t(1/2) = ln 2 / K

221s = ln 2 / K

K = ln 2 / 221s

K = 3.1364x10⁻³s⁻¹

If [A] = 1/64, [A]₀ = 1:

ln [A] = ln [A]₀ - kt

ln (1/64) = ln 1 - 3.1364x10⁻³t

4.1588 = 3.1364x10⁻³s⁻¹t

1326s = t

After 1326s, the concentration of pyruvic acid fall to 1/64 of its initial concentration.

The time takes for the concentration of pyruvic acid to fall to 1/64 of its initial value in this first order reaction 1326s.

How we calculate time?

Time will be calculated by using the first order kinetics equation as:

ln [A] = ln [A]₀ - kt, where

[A] = final concentration = 1/64

[A]₀ = initial concentration = 1

t = required time

k is the rate constant and will be calculated by using the half life duration 221s as:

t(1/2) = ln2 / k

221s = ln2 / k

k = ln2 / 221s = 3.1364x10⁻³ per sec

Now putting all these values on the above equation, we get

ln (1/64) = ln 1 - 3.1364x10⁻³t

4.1588 = 3.1364x10⁻³s⁻¹t

1326s = t

Hence, required time is 1326s.

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Skepticism _____. means not having an open mind is the belief that knowledge is not certain is a quality scientists should not have keeps scientists from making new discoveries

Answers

Answer:

(C) is the belief that knowledge is not certain.

Explanation: I would say it’s (C) because Most scientist or empirical skeptic is one who questions beliefs on the basis of scientific understanding and empirical evidence. Scientific skepticism may discard beliefs pertaining to purported phenomena not subject to reliable observation and thus not systematic or testable empirically.

Answer:

(C) is the belief that knowledge is not certain.

Explanation:

What is the mass of 5.80x10^23 atoms of silver

Answers

Answer:

5.80000000E+ 46...

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what is the name of the element that has electrons arranged
2,8,2 ​

Answers

Answer:

The metal element → Magnesium (Mg)

Answer:

magnesium

Explanation:

magnesium has electrons arranged 2,8,2

What volume of solution is prepared when 25.0 g of ethanol (C2H5OH) is added to enough water to make a 0.750 M ethanol solution?

Answers

Answer:

0.724 L

Explanation:

Step 1: Given data

Mass of ethanol (solute): 25.0 g

Concentration of the solution: 0.750 M

Step 2: Calculate the moles corresponding to 25.0 g of ethanol

The molar mass of ethanol is 46.07 g/mol.

25.0 g × (1 mol/46.07 g) = 0.543 mol

Step 3: Calculate the volume of the solution

We will use the following expression.

M = moles of solute / liters of solution

liters of solution = moles of solute / M

liters of solution = 0.543 mol / (0.750 mol/L)

liters of solution = 0.724 L

A 3.31-g sample of lead nitrate, Pb(NO3)2, molar mass = 331 g/mol, is heated in an evacuated cylinder with a volume of 2.53 L. The salt decomposes when heated, according to the equation:
2Pb(NO3)2(s)-->2PbO(s) + 4NO2(g) + O2(g)
Assuming complete decomposition, what is the pressure in the cylinder after decomposition and cooling to a temperature of 300. K? Assume the PbO(s) takes up negligible volume.

Answers

Answer:

0.486atm is the pressure of the cylinder

Explanation:

1 mole of Pb(NO₃)₂ descomposes in 4 moles of NO2 and 1 mole of O2. That is 1 mole descomposes in 5 moles of gas.

To find the pressure of the cylinder, we need to find moles of gas produced, and using general gas law we can determine the pressure of the gas:

Moles Pb(NO₃)₂ and moles of gas:

3.31g * (1mol / 331g) = 0.01 moles of Pb(NO₃)₂.

That means moles of gas produced is 0.05 moles.

Pressure of the gas:

Using PV = nRT

P = nRT/V

Where P is pressure (Incognite)

V is volume (2.53L)

R is gas constant (0.082atmL/molK)

T is absolute temperature (300K)

And n are moles of gas (0.05 moles)

P = 0.05mol*0.082atmL/molK*300K / 2.53L

P = 0.486atm is the pressure of the cylinder

What is the purpose of Oxidation?

Answers

Oxidation is when molecules r removed of hydrogen or combination with oxygen normally liberates large quantities of energy.
Oxidation is used in a wide variety of industries such as in the production of cleaning products and oxidizing ammonia to produce nitric acid, which is used in most fertilizers. Redox reactions are the foundation of electrochemical cells, which can generate electrical energy or support electrosynthesis.

Which one of the following combinations cannot function as a buffer solution?
A) HCN and KCN
B) NH3 and (NH4)2SO4
C) HNO3 and NaNO3
D) HF and NAF
E) HNO2 and NaNO2

Answers

Answer:

it is B

; )

Which one of the following combinations cannot function as a buffer solution?

A) HCN and KCN

B) NH3 and (NH4)2SO4

C) HNO3 and NaNO3

D) HF and NAF

E) HNO2 and NaNO2


What mainly inspired the Europeans to travel the ocean
1)colonization
2)trade
3)spread christianity
4)slavery

Answers

I would say 2)trade because every civilization will need to trade. You can’t find everything in one location.

What is the difference between an element and a compound?

A. An element is a pure substance, a compound is a mixture.

B. An element is more than one type of atom, a compound is a single type of atom.

C. An element is one type of atom, a compound is more than one type in mixed composition.

D. An element is one type of atom, a compound is more than one type bonded together.

Answers

D. Would be the correct answer :)
D.
im not completely sure but i did the process of elimination. it’s not B because an element is one type of atom. it’s not A because both elements and compounds are pure substances. and it’s not C because it’s not in mixed composition

in physics matter has three fundamental states gas solid and???

Answers

Liquid (Matter can exist in one of three main states: solid, liquid, or gas.)

Use the arrangement of particles in solids and gases to explain why solids are not as easy to compress as gases.

Answers

Answer:

The strong forces of attraction between the particles stop the particles from moving around each other and making the solid flow. The particles are close together so they need to be compressed hard to get them closer.

Explanation:

In solids, strong forces of attraction holds the particles together in a three-dimensional structure. The particles form an orderly arrangements called Lattice.

solids particles are all very close together that they’re touching. gasses particles are just floating around not touching

The only metal that exists as a liquid at room temperature is

Answers

Answer:

Mercury

Explanation:

This is the only metal that exists as a liquid at room temperature

Cylinders of compressed gas are typically filled to a pressure of 200 bar. For oxygen, what would be the molar volume at this pressure and 25 °C based on (i) the perfect gas equation, (ii) the van der Waals equation? For oxygen, a = 1.364 dm6 atm mol−2, b = 3.19 × 10−2 dm3 mol−1.

Answers

Answer:

a

 [tex]V  =   0.124 \ Liters[/tex]

b

[tex]V  =  0.112 \  Liters[/tex]

Explanation:

From the question we are told that

  The pressure of compressed gas is [tex]P  =  200 \  bar = \frac{200}{1.013}=  197.4 \ atm[/tex]

  The  temperature is  [tex]T  =  25^oC =  25 + 273 = 298 \  K [/tex]

Generally the perfect gas equation is mathematically represented as

     [tex]PV =  nRT[/tex]

substituting 0.08206 L-atm/mol-K  for R and  1 mole for n

We have that

     [tex]V  =  \frac{1 *  0.08206 * 298 }{ 197.4}[/tex]

       [tex]V  =   0.124 \ Liters[/tex]

Generally the van der Waals equation is mathematically represented as

       [tex]nRT  =  [P + \frac{n^2 * a }{V^2 } ][V - nb][/tex]

=>[tex]1 *  0.08206 *298  =  [197.4 + \frac{1^2 *  1.364}{V^2 } ][V - 1 * 3.19 * 10^{-2}][/tex]

=>    [tex]V  =  0.112 \  Liters[/tex]

Based upon the structures of acetylsalicylic acid, acetaminophen, and caffeine, including their functional groups and overall polarity, which best explains why ethanol is likely responsible for the fact that ASA and APHEN elute first in this solvent?

Answers

Answerb

Explanation:because I got it right

Compare the components that make up the windsurfer, his board, and his surroundings.

Answers

Answer:

The windsurfer, his board, and the air and water around him are all made of matter. That matter is made up of very small particles called atoms.

Explanation:

The windsurfer, the board, and the surroundings are all components involved in the activity of windsurfing. Let's compare them:

1. Windsurfer: The windsurfer is the person engaging in the sport. They are responsible for controlling the board and maneuvering through the water using the sail. The windsurfer's skills, balance, and physical abilities play a crucial role in their performance.

2. Board: The windsurfing board is the equipment used by the windsurfer. It typically consists of a long, narrow board with a fin at the bottom for stability and control. The board is designed to float on the water and provide a platform for the windsurfer to stand on.

3. Surroundings: The surroundings refer to the environment in which windsurfing takes place, which is usually a body of water such as a lake, river, or ocean. The condition of the water, including its temperature, depth, and currents, can affect the windsurfer's experience and performance.

In summary, the windsurfer, the board, and the surroundings are interconnected components of the windsurfing experience.

The skills and physical abilities of the windsurfer, the design and performance of the board, and the characteristics of the surrounding environment all contribute to the overall enjoyment and success of the activity.

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Of 4,500 customers last month, 710 ordered items from the lighter menu selections. Whatpercent is this? What type of information does a physical map show? Excerpt from Areojet Corporation records for month of February: Per Unit Per Month Selling price $ 200,000 Direct materials used in production 40,000 Direct labor 10,000 Variable manufacturing overhead 2,000 Fixed manufacturing overhead $ 140,000 Variable selling and administrative expenses 20,000 Fixed selling and administrative expenses 40,000 Assuming the absorption costing method is used, what is the total manufacturing costs added to work in process during the month of February Which source is more credible for your research and why in windows 10, what is the name of a program you can use to find a file or folder? If DM = 25, what is the value of r? A.10B.12C.14 D.15 What evidence is a blood smear on a car How does a person's response to stress change throughout all three phases of Selye's general adaptation syndrome? Answer: In the first phase of the general adaptation response (the alarm phase), the body reacts to a stressor with an alarm reaction. A person may notice a quickening heart rate and an increase in breathing and energy. In the second stage of the general adaptation response (the resistance phase), the body releases hormones to stabilize body, but the body still remains at high alert as physiological responses remain elevated. Finally, the body reaches exhaustion phase if the stress continues. In this phase, the person has used all resources and has nothing more to use to cope with the stressor. It is during this stage that the person's emotional and physical health begins to decline. What was the main purpose of the TownShend Act is -18.75 rational or irrational number Choices: True, False. Select all that are True.ON2 means that each nitrogen atom is separate from each other (not bonded).N2 means that each nitrogen atom is bonded to another and they act as a unit.2N is considered a molecule whereas N2 is not.2N means that each nitrogen atom is separate from the other (not bonded).2N means that each nitrogen atom is bonded to another and they act as a unit. Will vote brainliest!!!!!!!! Thank you!!!!!!!!!!!! Iff(x) = 2x2 - 5andg(x) = 5x +7Findg(f(x)) = [? ]x2 +Help pls Leon bought a dozen daisies for $3.75. To the nearest hundredth, approximately how much did Leon pay for each daisy? 2. In the following sentence, which of the words listed is a COLLECTIVENOUN? Gracie and will baked a batch of fresh cookies for their mom. *.A. GracieB. WillC. batchD. mom 6+4/a+b/3 when a=4 and b=3 plzzzzzz helpppp meeeee aaaassssaaappppp Give an example of when an actual net income would result in a negative number.a. When the budgeted expenses exceed the actual expenses, the result is a negative actualnet incomeb. When the actual expenses exceed the actual income, the result would be a negative dollaramount.C. When the budgeted income is less than the actual income, the result is a negative actual netincomed. When the actual expenses are less than the actual income, the result is a negative dollaramount.Please select the best answer from the choices provided A bookkeeper records a positive number to show when a sale is made at a store and a negativenumber to show when the store has a cost. Which equation could represent how thebookkeeper records the store having 4 shipping costs of $19.42? Enter the coordinates of the image under the transformation (6, -12).(x, y)- (x +Y-(1,-4)>(8, -13)(12, -12)(