Answer:
The list of ingredients are used in this method are as follow-
1. Acrylamide - mostly 30%
2. Bisacrylamide - mostly 0.8%
3. 1.5M Tris-Hcl pH 6.8 for stacking gel and 8.8 for separating gel.
4. 10% SDS
5. 10% APS
6. TEMED
7. Double distilled water
Polymerization:
Acrylamide is a polymer that is used to dissolved in buffer and bisacrylamide is the cross linking agen.
Two types of gels are prepared for separation of protein. Stacking gel is usually 4 or 6% and separating gel is usually 10 to 20%. Lower concentration of stacking gel will allow the migration of all the protein same because of larger pore size. It will stack or line up the proteins for its next level of separation.
Higher concentration of separating gel will provide resistance to the movement of protein because of the low pore size and therefore linear bands can be achieved neatly.
A. Form a new question that relates to the interaction of organisms in the ecosystem.
Answer:Predation, parasitism and herbivory are the three interactions which are found in different organisms of an ecosystem.
Lion is a predator which feed on flesh of other animals such as herbivores.
Explanation:
Guys how to convince my dad to give me money
Answer:
hey dad can i have some money
if he says no
then no
Explanation:
Answer:
First ask for a small amount than right after he gives you the small amount ask for a bigger amount
This trick works all the time
Explanation:
this is my question
Answer: B
Explanation: im sorry if this is wrong
Have a great day.
b) What is the name of the tube that connects the liver to the alimentary canal?
Answer:
Tube is a connected liver alimentary canal
Explanation:
Tube id miob
diegetic sound in the film monkeys paw 2011
Antibodies, lymphocytes, B
cells, and T cells are all a part
of which type of immune
system defense?
Answer:
A
Explanation:
specific immune defense system
A hole in the skin causes platelets to
rush to the site and release a chemical
that releases more platelets. This is an
example of what type of mechanism?
Answer:
b number positive feedback
"A hole in the skin causes platelets to rush to the site and release a chemical that releases more platelets" is an example of a positive feedback mechanism. The correct option is B.
What is a positive feedback mechanism?A positive feedback mechanism is a technique of the body in which the body works or secretes something to the body that is needed more in the body, and it is secreted in the body out of league and number.
Platelets are present in the blood. They are spherical and are colorless and small. They help in blood clotting when there is a cut or bleeding, it prevents loss of blood by clotting the blood.
So, in the hole of the body, there will be blood loss, and releasing more platelets would help in blood clotting.
Thus, the correct option is B. positive feedback mechanism.
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John had unattached earlobes like his father, but his mother has attached earlobes. What is johns genotype with unattached being dominant
Genotype refers to the complete genetic make-up and can also be referred to as the alleles an organism carries on a particular gene.
John's genotype with unattached being dominant is Aa.
Using A to represent the allelesSince his mother has attached earlobes, her condition can only be inherited in the recessive condition (aa). So for John to have an unattached earlobe, his father has to be either AA or Aa. Therefore John's genotype would be AaLearn more about genotype: https://brainly.com/question/22117
g persons with the inherited disease familial hypercholesterolemia have very high levels of cholestoral because of
Answer:
a genetic mutation passed on by one or two of their parents.
Explanation:
please help :(
Which of the following defines a cash crop?
any crop grown to be sold for direct sale
any crop grown to be sold to other growers
any crop grown to be sold as a raw material
any crop grown to be sold on the commodities market
Answer:
Any crop grown to be sold for Direct sale A cash crop or profit crop is an agricultural crop which is grown to sell for profit. ... The term is used to differentiate marketed crops from subsistence crops, which are those fed to the producer's own livestock or grown as food for the producer's family.Hope this helps you!!
Anything made from crude oil or natrual gas can be made from synthetic oil.
True
Or
False
Answer: True but not always
Use the story below to fill in the questions.
A) Origin and development
B) Earth
C) Life
D) Early technologies
Answer:
letter A
Explanation:
because the story about the creation of the Maya Narration is discuss about the past world earth who there was no light,no electricity,no cellphone,no have technologies and it's silence after so many people have come and birth in the world,so many changes have done,we have now connection in our society in social and in reality,that ultimately is producing true,and enduring.
Can Glucose turn into fat
Answer:
yea
Explanation:
If u consume to much or have diabetes its cant run it so it turns into fat
which is true about a hypotonic solution? a. it has a higher concentration of wtwr and a lower concentration of solutes than another solution
b. ut has a higher concentration of solutes and a lower concentration of water than other solution
c. it has an equal concentration of water and solutes as another solution
d. it has more solutes than solvent than another solution
Answer:
A
Explanation:
A hypotonic solution has a lower concentration of solutes than another solution, but has more water.
Could there be winds without differential heating? Using evidence from Unit 5 explain what differential heating is and whether this effects the earth’s global wind patterns.
HELPPP PLEASE SITE EVIDENCE (WILL MARK BRAINLIEST)!!!!!!!!
Differential heating is responsible for the wind patterns across the globe.
Differential heating is defined as the difference in heating of air on the land versus water. Sea breezes occur when land heats up faster than water which rises and creates a low pressure system. The high pressure air from the sea influxes is responsible for the creation of a sea breeze.
Unequal heating of the Earth's surface is responsible for the formation of large global wind patterns. Warm air rises at the equator and moves toward the poles while on the other hand, the cooler air rises at the poles and moves back toward the equator so in this way the earth’s global wind patterns occurs so we can say that differential heating is responsible for the wind patterns across the globe.
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PLEASE HELP!! 70 POINTS!!!
Explain how DNA is involved in creating genetic variation within a species. Is this considered a mutation, if so why?
Answer:
different strands creates different human characteristic
When might you take a deep breath?
Answer:
When you're stressed??
Explanation:
i don't really know I never learned this, but I hope this helps
Answer:
In fresh morning......or when ur stressed......or simply when u feel like .......
A mother has blood type A and her son has blood type O. Which blood types are possible for the father?
what is Nuclear Fusion.
Answer: Nuclear Fusion occurs when the nuclear components of an atom combine.
what determines which individuals will survive and which will not? help me please I hate biology so much
Explanation:
pretty sure the answer is, "adaptation."
only the creatures that can adapt 2 changing environments are the ones who survive...
If you gently bend your ear, and then let go, the shape of your ear will return because the cartilage of your ear contains_____.
Answer:
If you gently bend your ear, and then let go, the shape of your ear will return because the cartilage of your ear contains elastic fibers.
Select all that apply. Hair infections are generally
caused by
bacteria.
fungi.
viruses.
ODO
cancer.
lice.
DONE
Answer: A. Bacteria, B. Fungi, E. Lice.
Explanation:
How do bogs keep themselves cool
Answer: Like humans, dogs need to keep themselves cool to protect their bodies. They do sweat, but not in the same way. They only have sweat glands only on the paw pads and nose leather. Since they are covered with fur, it is more challenging to keep their bodies cool.
After your dog runs around, you will notice that he begins to pant heavily. Dogs rely on panting to keep their bodies cool. Panting causes the evaporation of water from the nasal cavity, tongue, throat and lungs, which helps lower their body temperature.
They also experience the same dilation in blood vessels as humans, which brings blood to the surface of the skin, allowing the air in the environment to cool them.
Any dog is at risk for heat-related problems, especially in warm, humid weather. Our brachycephalic friends are at a much greater risk than their long-nosed counterparts. Large breeds with thick coats, such as Bernese Mountain dogs or Pyrenees are also more prone. Dogs with pre-existing heart conditions or respiratory issues are also more likely to be overcome by the heat.
When these dogs pant to try and cool themselves, they have to work harder to do so. This only raises their body temperature even higher, making it worse instead of better.
- Psychology PLS HELP-
Tom's directive and confrontive therapist challenges Tom's belief that he is worthless because he is not the
world's greatest Superhero. Tom's therapist is most likely:
A. an eclectic therapist
B. a psychoanalyst like Sigmund Freud
C. a cognitive therapist like Aaron Beck
D. a humanistic therapist like Carl Rogers
E. a humanistic, client-centered therapist
Tom's therapist is most likely an eclectic therapist.
What is eclectic therapy?Eclectic therapy is a method that draws on orientation and many theoretical approaches. It is a flexible and multi-faceted treatment that allows the therapist to use the most effective methods available to address the needs of each client.
What is an example of eclectic therapy?An example of an eclectic program is where children spend part of each day experiencing a variety of treatments, such as formal teaching using behavioral analytical techniques (ABA), sensory stimulation (brushing and flipping), down-to-earth routines, and music sessions, and free play with regular peers.
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In translation What are are the three letters called in mRNA and what are they connected to ??
Answer:
During translation, the ribosome reads 3 letters at a time on the mRNA strand called a codon. The anticodons, which are connected to the amino acid, match up with the letters on the mRNA strand
Explanation:
Solar radiation can cause skin cancer in an individual. If a man with skin cancer caused by solar radiation has offspring, which of the following statements would be true? A. only the man getting skin cancer would be affected B. only the man and his male offspring would be affected C. only the man and his female offspring would be affected D. the man and all of his offspring would be affected
when did large mouth bass arrive in nebraska
Explanation:
around 1880 and 1883
i think
Base your answer(s) to the following question on the diagrams below and your knowledge of biology. The diagrams represent two different cells and some of their parts. The diagrams are not drawn to scale. Which statement best describes these cells?
Answer:
The diagrams represent two different cells and some of their parts. The diagrams are not drawn to scale.
Explanation:
Read the following passage and answer the question.
“Never another master kind as he!
I’ll never find one – no matter where I go,
not even if I went back to mother and father,
the house where I was born and my parents reared me once.
Ah, but much as I grieve for them, much as I long
to lay my eyes on them. Set food on the old soil,
it’s longing for him, him that wrings my heart –
Odysseus, lost and gone!
That man, old friend, far away as he is…
I can scarcely bear to say his name aloud,
so deeply he loved me, cared for me, so deeply.
Worlds away as he is, I call him my Master, Brother!”
Which theme is being expressed in this passage?
hospitality
temptation
pride
loyalty
Answer:
it's like two theme there
Explanation:
hospitality or loyalty
What would happen if any of the biogeochemical cycles would break
Answer:
Excess phosphorus is polluting our environment while, ironically, mineable resources of this essential nutrient are limited. Globally, oxygen-depleted marine coastal 'dead zones' caused by nutrient-stimulated algal blooms continue to expand
Explanation:
